Optimization of a laboratory-developed test utilizing roche analyte-specific reagents for detection of Staphylococcus aureus, methicillin-resistant S. aureus, and vancomycin-resistant Enterococcus species

doi:10.1128/JCM.00230-08

. 2008 Jul;46(7):2377-80.

doi: 10.1128/JCM.00230-08. Epub 2008 Apr 30.

Optimization of a laboratory-developed test utilizing roche analyte-specific reagents for detection of Staphylococcus aureus, methicillin-resistant S. aureus, and vancomycin-resistant Enterococcus species

Maitry S Mehta¹, Suzanne M Paule, Donna M Hacek, Richard B Thomson, Karen L Kaul, Lance R Peterson

Affiliations

PMID: 18448688
PMCID: PMC2446926
DOI: 10.1128/JCM.00230-08

Optimization of a laboratory-developed test utilizing roche analyte-specific reagents for detection of Staphylococcus aureus, methicillin-resistant S. aureus, and vancomycin-resistant Enterococcus species

Maitry S Mehta et al. J Clin Microbiol. 2008 Jul.

. 2008 Jul;46(7):2377-80.

doi: 10.1128/JCM.00230-08. Epub 2008 Apr 30.

Authors

Maitry S Mehta¹, Suzanne M Paule, Donna M Hacek, Richard B Thomson, Karen L Kaul, Lance R Peterson

Affiliation

¹ Evanston Northwestern Healthcare, Evanston, Illinois 60201, USA.

PMID: 18448688
PMCID: PMC2446926
DOI: 10.1128/JCM.00230-08

Abstract

Nasal and perianal swab specimens were tested for detection of Staphylococcus aureus and vancomycin-resistant Enterococcus species (VRE) using a laboratory-developed real-time PCR test and microbiological cultures. The real-time PCR and culture results for S. aureus were similar. PCR had adequate sensitivity, but culture was more specific for the detection of VRE.

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References

1. Anderson, D. J., D. J. Sexton, Z. A. Kanafani, G. Auten, and K. S. Kaye. 2007. Severe surgical site infection in community hospitals: epidemiology, key procedures, and the changing prevalence of methicillin-resistant Staphylococcus aureus. Infect. Control Hosp. Epidemiol. 281047-1053. - PubMed
1. Biedenbach, D. J., G. J. Moet, and R. N. Jones. 2004. Occurrence and antimicrobial resistance pattern comparisons among bloodstream infection isolates from the SENTRY Antimicrobial Surveillance Program (1997-2002). Diagn. Microbiol. Infect. Dis. 5059-69. - PubMed
1. Clinical and Laboratory Standards Institute. 2006. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved standard, 7th edition. Clinical and Laboratory Standards Institute, Wayne, PA.
1. Hacek, D. M., S. Paule, M. Small, R. Gottschall, R. Thomson, and L. Peterson. 2003. Abstr. 103rd Gen. Meet. Am. Soc. Microbiol., abstr. C-323.
1. Hacek, D. M., W. J. Robb, S. M. Paule, J. C. Kudrna, V. P. Stamos, and L. R. Peterson. 2008. Staphylococcus aureus nasal decolonization in joint replacement surgery reduces infection. Clin. Orthop. Relat. Res. [Epub ahead of print.] doi:10.1007/s11999-008-0210-y. - DOI - PMC - PubMed

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[1] Anderson, D. J., D. J. Sexton, Z. A. Kanafani, G. Auten, and K. S. Kaye. 2007. Severe surgical site infection in community hospitals: epidemiology, key procedures, and the changing prevalence of methicillin-resistant Staphylococcus aureus. Infect. Control Hosp. Epidemiol. 281047-1053. - PubMed

[2] Anderson, D. J., D. J. Sexton, Z. A. Kanafani, G. Auten, and K. S. Kaye. 2007. Severe surgical site infection in community hospitals: epidemiology, key procedures, and the changing prevalence of methicillin-resistant Staphylococcus aureus. Infect. Control Hosp. Epidemiol. 281047-1053. - PubMed

[3] Biedenbach, D. J., G. J. Moet, and R. N. Jones. 2004. Occurrence and antimicrobial resistance pattern comparisons among bloodstream infection isolates from the SENTRY Antimicrobial Surveillance Program (1997-2002). Diagn. Microbiol. Infect. Dis. 5059-69. - PubMed

[4] Biedenbach, D. J., G. J. Moet, and R. N. Jones. 2004. Occurrence and antimicrobial resistance pattern comparisons among bloodstream infection isolates from the SENTRY Antimicrobial Surveillance Program (1997-2002). Diagn. Microbiol. Infect. Dis. 5059-69. - PubMed

[5] Clinical and Laboratory Standards Institute. 2006. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved standard, 7th edition. Clinical and Laboratory Standards Institute, Wayne, PA.

[6] Clinical and Laboratory Standards Institute. 2006. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved standard, 7th edition. Clinical and Laboratory Standards Institute, Wayne, PA.

[7] Hacek, D. M., S. Paule, M. Small, R. Gottschall, R. Thomson, and L. Peterson. 2003. Abstr. 103rd Gen. Meet. Am. Soc. Microbiol., abstr. C-323.

[8] Hacek, D. M., S. Paule, M. Small, R. Gottschall, R. Thomson, and L. Peterson. 2003. Abstr. 103rd Gen. Meet. Am. Soc. Microbiol., abstr. C-323.

[9] Hacek, D. M., W. J. Robb, S. M. Paule, J. C. Kudrna, V. P. Stamos, and L. R. Peterson. 2008. Staphylococcus aureus nasal decolonization in joint replacement surgery reduces infection. Clin. Orthop. Relat. Res. [Epub ahead of print.] doi:10.1007/s11999-008-0210-y. - DOI - PMC - PubMed

[10] Hacek, D. M., W. J. Robb, S. M. Paule, J. C. Kudrna, V. P. Stamos, and L. R. Peterson. 2008. Staphylococcus aureus nasal decolonization in joint replacement surgery reduces infection. Clin. Orthop. Relat. Res. [Epub ahead of print.] doi:10.1007/s11999-008-0210-y. - DOI - PMC - PubMed

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Optimization of a laboratory-developed test utilizing roche analyte-specific reagents for detection of Staphylococcus aureus, methicillin-resistant S. aureus, and vancomycin-resistant Enterococcus species

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Optimization of a laboratory-developed test utilizing roche analyte-specific reagents for detection of Staphylococcus aureus, methicillin-resistant S. aureus, and vancomycin-resistant Enterococcus species

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