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. 2008;59(8):2277-84.
doi: 10.1093/jxb/ern098. Epub 2008 May 2.

Rice-arsenate interactions in hydroponics: a three-gene model for tolerance

Gareth J Norton  1 , Meher Nigar, Paul N Williams, Tapash Dasgupta, Andrew A Meharg, Adam H Price
Affiliations

Rice-arsenate interactions in hydroponics: a three-gene model for tolerance

Gareth J Norton et al. J Exp Bot. 2008.

Abstract

In this study, the genetic mapping of the tolerance of root growth to 13.3 muM arsenate [As(V)] using the BalaxAzucena population is improved, and candidate genes for further study are identified. A remarkable three-gene model of tolerance is advanced, which appears to involve epistatic interaction between three major genes, two on chromosome 6 and one on chromosome 10. Any combination of two of these genes inherited from the tolerant parent leads to the plant having tolerance. Lists of potential positional candidate genes are presented. These are then refined using whole genome transcriptomics data and bioinformatics. Physiological evidence is also provided that genes related to phosphate transport are unlikely to be behind the genetic loci conferring tolerance. These results offer testable hypotheses for genes related to As(V) tolerance that might offer strategies for mitigating arsenic (As) accumulation in consumed rice.

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Figures

Fig. 1.
Fig. 1.
Arsenate uptake of root tips after 20 min in a range of arsenate solutions. Filled symbols and the solid line are Azucena, and open symbols and the dashed line are Bala. The bar is the standard error (n=3).
Fig. 2.
Fig. 2.
Arsenate tolerance of Azucena (filled bars) and Bala (open bars) at a range of phosphate concentrations in 13.3 μM (upper) and 133 μM (lower) arsenate. The error bar is the standard error.
Fig. 3.
Fig. 3.
Tolerance to 6.65 μM arsenate of Azucena (filled bars) and Bala (open bars) which had previously been grown for a week in nutrient solution with or without 0.3 mM phosphate. The error bar is the standard error.
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