Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1991 Jan;65(1):308-12.
doi: 10.1128/JVI.65.1.308-312.1991.

Mutants of feline immunodeficiency virus resistant to 3'-azido-3'-deoxythymidine

K M Remington  1 , B Chesebro, K Wehrly, N C Pedersen, T W North
Affiliations

Mutants of feline immunodeficiency virus resistant to 3'-azido-3'-deoxythymidine

K M Remington et al. J Virol. 1991 Jan.

Abstract

We selected 3'-azido-3'-deoxythymidine (AZT)-resistant mutants of feline immunodeficiency virus (FIV) in a cat cell culture system. The characterization of one of these mutants was facilitated by the development of a focal immunoassay which could accurately measure FIV infectivity. This assay was used to quantitate the susceptibility of FIV to various inhibitors. The AZT-resistant mutant was found to be cross-resistant to 3'-azido-2',3'-dideoxyuridine and 3'-azido-2',3'-dideoxyguanosine but remained sensitive to several other inhibitors (2',3'-dideoxyinosine, 2',3'-dideoxy-2',3'-didehydrothymidine, and phosphonoformate). These patterns of cross-resistance and sensitivity were similar to those of the AZT-resistant human immunodeficiency virus (HIV) that has recently been isolated from patients with AIDS (B. A. Larder and S. D. Kemp, Science 246:1155-1158, 1989). Like the AZT-resistant HIV, purified reverse transcriptase from mutant FIV failed to show resistance to the 5'-triphosphate of AZT. This mutant can be used in the FIV model system to study the mechanisms of drug resistance and to determine the pathogenicity of AZT-resistant mutants.

PubMed Disclaimer

References

    1. Science. 1989 Dec 1;246(4934):1155-8 - PubMed
    1. Proc Natl Acad Sci U S A. 1989 Jul;86(13):4803-7 - PubMed
    1. Am J Vet Res. 1988 Aug;49(8):1246-58 - PubMed
    1. Proc Natl Acad Sci U S A. 1985 Oct;82(20):7096-100 - PubMed
    1. Nature. 1987 Jun 25-Jul 1;327(6124):716-7 - PubMed

Publication types