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. 1991 Jan;173(2):896-9.
doi: 10.1128/jb.173.2.896-899.1991.

Transposon Tn5supF-based reverse genetic method for mutational analysis of Escherichia coli with DNAs cloned in lambda phage

S H Phadnis  1 , S Kulakauskas, B R Krishnan, J Hiemstra, D E Berg
Affiliations

Transposon Tn5supF-based reverse genetic method for mutational analysis of Escherichia coli with DNAs cloned in lambda phage

S H Phadnis et al. J Bacteriol. 1991 Jan.

Abstract

An efficient method for systematic mutational analysis of the Escherichia coli genome was developed. It entails Tn5supF transposition to lambda-E. coli hybrid phage clones (Kohara library) and then transduction of recipient cells to Sup+. Essential and nonessential genes are distinguished by the ability of insertion mutant phage to form haploid versus only heterozygous partial diploid bacterial recombinants.

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