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Comparative Study
. 2008 Jul;46(7):2195-9.
doi: 10.1128/JCM.00315-08. Epub 2008 May 7.

Comparison of the NucliSens easyMAG and Qiagen BioRobot 9604 nucleic acid extraction systems for detection of RNA and DNA respiratory viruses in nasopharyngeal aspirate samples

Kwok Hung Chan  1 , Wing Cheong Yam, Chiu Mei Pang, Kit Man Chan, Siu Yan Lam, Kam Fai Lo, Leo L M Poon, J S Malik Peiris
Affiliations
Comparative Study

Comparison of the NucliSens easyMAG and Qiagen BioRobot 9604 nucleic acid extraction systems for detection of RNA and DNA respiratory viruses in nasopharyngeal aspirate samples

Kwok Hung Chan et al. J Clin Microbiol. 2008 Jul.

Abstract

The NucliSens easyMAG and BioRot 9604 automated nucleic acid extraction systems were evaluated and compared with the manual QIAamp (Qiagen) extraction method for their abilities to extract nucleic acid from nasopharyngeal aspirate samples for the detection of RNA and DNA respiratory viruses. The nucleic acids recovered by all three methods gave comparable sensitivities in PCR tests, and the three methods gave comparable viral loads. There was no evidence of residual PCR inhibitors and no evidence of PCR cross-contamination.

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Figures

FIG. 1.
FIG. 1.
Comparison of viral loads of influenza A virus (a), RSV (b), and adenovirus (c) in nucleic acid extracted from NPA specimens by the automated easyMAG system and BioRobot 9604 kit and the manual Qiagen RNA kit.
See this image and copyright information in PMC

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