Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1991 Feb;173(3):1096-104.
doi: 10.1128/jb.173.3.1096-1104.1991.

Transposition of Tn5096 and other IS493 derivatives in Streptomyces griseofuscus

P J Solenberg  1 , R H Baltz
Affiliations

Transposition of Tn5096 and other IS493 derivatives in Streptomyces griseofuscus

P J Solenberg et al. J Bacteriol. 1991 Feb.

Abstract

Tn5096 was constructed by inserting an apramycin resistance gene, aac(3)IV, into IS493 from Streptomyces lividans. By using conventional and pulsed-field gel electrophoresis, Tn5096 and related transposons were shown to insert into many different locations in the Streptomyces griseofuscus chromosome and in two linear plasmids. On insertion into the target site CANTg, 3 bp appeared to be duplicated. Independent transpositions were obtained by delivery of the transposon from a temperature-sensitive plasmid. The frequency of auxotrophy among cultures containing transpositions was about 0.2%.

PubMed Disclaimer

References

    1. J Antibiot (Tokyo). 1990 Sep;43(9):1204-5 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 - PubMed
    1. J Bacteriol. 1988 Apr;170(4):1955-7 - PubMed
    1. Mol Gen Genet. 1985;200(2):235-9 - PubMed
    1. Mol Gen Genet. 1986 Apr;203(1):79-88 - PubMed

LinkOut - more resources