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. 1998;43(2):161-8.
doi: 10.1007/BF02816503.

Laboratory-scale production of lignin-degrading enzymes by free and entrapped cells ofPhanerochoete chrysosporium in a tubular air-lift bioreactor

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Laboratory-scale production of lignin-degrading enzymes by free and entrapped cells ofPhanerochoete chrysosporium in a tubular air-lift bioreactor

I Darah et al. Folia Microbiol (Praha). 1998.

Abstract

The production of lignin-degrading enzymes by free and entrapped cells ofPhanerochoete chrysosporium in a tubular air-lift bioreactor was studied. Under optimized cultural conditions the production of lignin peroxidase by free cells, calcium-alginate-entrapped cells and scouring-mesh-entrapped cells was in a ratio of 520ratio720ratio950 mU/mL, while the production of manganese peroxidase was in a ratio of 350ratio480ratio620 mU/mL. The stability of the entrapped cells by fed-batch systems was highest after 3 feeding experiments which is similarly demonstrated in the repeated use of the preparations in batch system.

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