The amino-terminal 147 amino acids of SV40 large T antigen transform secondary rat embryo fibroblasts

Abstract

T147D is an SV40 mutant that encodes only the amino-terminal 147 amino acids of large T antigen and does not make small t antigen. We have constructed a recombinant retrovirus that expresses the T147D mutant protein. We show here that this virus can transform the established rat cell line, F111, in an agar assay with high efficiency. More importantly, we demonstrate that this retrovirus transforms secondary rat embryo fibroblasts to anchorage independence as efficiently as a recombinant retrovirus that expresses both wild-type large and small T antigens. These data indicate that in rat cells, the amino-terminal 147 amino acids of T antigen are sufficient for transformation. Further, since the T147D protein does not bind p53, we conclude that the association between T antigen and p53 is not required for the transformation of rat cells to anchorage-independent growth.