[Filamentous fungi preservation in distilled water]

Abstract

Some methods for keeping the fungal Culture Collection have been used. However, the choice of either one on the basis that must ensure the cultural genetic stability and its phenotipic characteristics. In this work the preservation method in distilled water recognized in the literature as a single, economic and certain method that guarantie the survival of fungus cultures for long periods was used. 26 strains of generus and species: Aspergillus niger, Aspergillus candidus, Fusarium sp., Fusarium moniliforme, Mucor griseocyanum, Syncephalastrum sp., Trichoderma sp., Trichoderma harzianum and Trichoderma koningii were preserved. Enough inoculum from well developed cultures (mainly spores and hyphae) poured in flasks with sterile distilled water warranted a 100% of survival of those microorganisms for two years. At the same time no apparent changes were observed in respect to morphology and macroscopic features.