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. 1991 Mar;11(3):1538-46.
doi: 10.1128/mcb.11.3.1538-1546.1991.

Identification of a cis-acting sequence required for germ line-specific splicing of the P element ORF2-ORF3 intron

A C Chain  1 , S Zollman, J C Tseng, F A Laski
Affiliations

Identification of a cis-acting sequence required for germ line-specific splicing of the P element ORF2-ORF3 intron

A C Chain et al. Mol Cell Biol. 1991 Mar.

Abstract

P element transposition in Drosophila melanogaster is limited to the germ line because the third intron (the ORF2-ORF3 intron) of the P element transcript is spliced only in germ line cells. We describe a systematic search for P element sequences that are required to regulate the splicing of the ORF2-ORF3 intron. We have identified three adjacent mutations that abolish the germ line specificity and allow splicing of this intron in all tissues. These mutations define a 20-base regulatory region located in the exon, 12 to 31 bases from the 5' splice site. Our data show that this cis-acting regulatory sequence is required to inhibit the splicing of the ORF2-ORF3 intron in somatic cells.

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