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. 1991 Mar;11(3):1759-64.
doi: 10.1128/mcb.11.3.1759-1764.1991.

Induction of a cyclic AMP-responsive gene in living cells requires the nuclear factor CREB

J L Meinkoth  1 , M R Montminy, J S Fink, J R Feramisco
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Induction of a cyclic AMP-responsive gene in living cells requires the nuclear factor CREB

J L Meinkoth et al. Mol Cell Biol. 1991 Mar.

Abstract

We constructed cell lines containing various enhancer elements cloned upstream from a marker gene. By microinjection of specific antibodies directed against transcriptional activator proteins into these cell lines, we have developed a functional assay for factors which regulate the activity of target promoters. Here we show that microinjection of a highly specific antibody to the cyclic AMP enhancer element-binding (CREB) protein diminishes gene expression in response to cyclic AMP in living fibroblasts.

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