Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2008 Jul;26(6):721-6.
doi: 10.1016/j.mri.2008.01.002. Epub 2008 May 13.

Feasibility of using hyperpolarized [1-13C]lactate as a substrate for in vivo metabolic 13C MRSI studies

Albert P Chen  1 , John Kurhanewicz, Robert Bok, Duan Xu, David Joun, Vickie Zhang, Sarah J Nelson, Ralph E Hurd, Daniel B Vigneron
Affiliations

Feasibility of using hyperpolarized [1-13C]lactate as a substrate for in vivo metabolic 13C MRSI studies

Albert P Chen et al. Magn Reson Imaging. 2008 Jul.

Abstract

The development of dynamic nuclear polarization in solution has enabled in vivo 13C MR studies at high signal-to-noise ratio following injection of prepolarized 13C substrates. While prior studies have demonstrated the ability to observe metabolism following injection of hyperpolarized 13C pyruvate, the goal of this study was to develop and test a new hyperpolarized agent for investigating in vivo metabolism, [1-13C]lactate. A preparation for prepolarized 13C lactate and the requisite dissolution media were developed to investigate the feasibility for in vivo 13C MRS/MRSI studies following injection of this hyperpolarized agent. This study demonstrated, for the first time, not only the ability to detect hyperpolarized [1-13C]lactate in vivo but also the metabolic products 13C pyruvate, 13C alanine and 13C bicarbonate following injection in normal rats. The use of 13C lactate as a substrate provided the opportunity to study the conversion of lactate to pyruvate in vivo and to detect the secondary conversions to alanine and bicarbonate through pyruvate. This study also demonstrated the potential value of this hyperpolarized agent to investigate in vivo lactate uptake and metabolism in preclinical animal models.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Dynamic MRS data from a normal rat after injection of hyperpolarized 13C lactate. Both a stacked plot of individual spectra from each time points (first 25 time points, top right) and a time course graph (bottom left) of the 13C lactate, 13C alanine and 13C pyruvate peak amplitude were shown.
Figure 2
Figure 2
Stacked plot of in vivo dynamic MRS spectra (first 16 time points, a) as well as a summed spectrum from the stacked shown (b). In addition to the 13C lactate, alanine and pyruvate resonances, the 13C bicarbonate resonance was also observed.
Figure 3
Figure 3
Axial T2-weighted anatomical image (left) from a rat through one of the kidneys as well as the corresponding 13C MRSI data (right) are shown. High level of 13C lactate signal was observed throughout the slice, 13C alanine and pyruvate resonances were observed in voxels containing kidney and voxels containing muscle and vasculature.
See this image and copyright information in PMC

References

    1. Ardenkjaer-Larsen JH, Fridlund B, Gram A, Hansson G, Hansson L, Lerche MH, Servin R, Thaning M, Golman K. Increase in signal-to-noise ratio of > 10,000 times in liquid-state NMR. Proc Natl Acad Sci U S A. 2003;100(18):10158–10163. - PMC - PubMed
    1. Golman K, Ardenkjaer-Larsen JH, Peterson JS, Mansson S, Leunbach I. Molecular Imaging with endogenous substances. Proc Natl Acad Sci U S A. 2003;100(18):10435–10439. - PMC - PubMed
    1. Golman K, Zandt R, Thaning M. Real-time metabolic imaging. PNAS. 2006;103(30):11270–11275. - PMC - PubMed
    1. Chen AP, Alberts MJ, Cunningham CH, Kohler SJ, Yen Y, Hurd RE, Tropp J, Bok R, Pauly JM, Nelson SJ, Kurhanewicz J, Vigneron DB. Hyperpolarized C-13 Spectroscopic Imaging of the TRAMP Mouse at 3T – Initial Experience. Magn Reson Med. In-Press. - PubMed
    1. Kohler SJ, Yen Y, Wolber J, Chen AP, Albers MJ, Bok R, Zhang V, Tropp J, Nelson S, Vigneron DB, Kurhanewicz J, Hurd RE. In vivo 13 carbon metabolic imaging at 3T with hyperpolarized 13C-1-pyruvate. Mag Res Med. 2007;58(1):65–69. - PubMed

Publication types