Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 2008 Jun;9(2):169-75.
doi: 10.4142/jvs.2008.9.2.169.

In vivo morphological and antigenic characteristics of Photobacterium damselae subsp. piscicida

Tae S Jung  1 , Kim D Thompson, Donatella Volpatti, Marco Galeotti, A Adams
Affiliations
Comparative Study

In vivo morphological and antigenic characteristics of Photobacterium damselae subsp. piscicida

Tae S Jung et al. J Vet Sci. 2008 Jun.

Abstract

The present study was conducted to examine the morphology and antigenicity of Photobacterium damselae subsp. piscicida by culturing the bacterium in vivo in the peritoneal cavity of sea bass (Dicentrarchus labrax) within dialysis bags with either a low molecular weight (LMW) cut-off of 25 kDa or a high molecular weight (HMW) cut-off of 300 kDa. Differences were observed in the growth rate between the bacteria cultured in vivo or in vitro. Bacteria cultured in vivo were smaller and produced a capsular layer, which was more prominent in bacteria cultured in the HMW bag. Antigenicity was examined by Western blot analysis using sera from sea bass injected with live Ph. d. subsp. piscicida. The sera recognised bands at 45 and 20 kDa in bacteria cultured in vivo in the LMW bag. Bacteria cultured in vivo in the HMW bag did not express the 45 kDa band when whole cell extracts were examined, although the antigen was present in their extracellular products. In addition, these bacteria had a band at 18 kDa rather than 20 kDa. Differences in glycoprotein were also evident between bacteria cultured in vitro and in vivo. Bacteria cultured in vitro in LMW and HMW bags displayed a single 26 kDa band. Bacteria cultured in the LMW bag in vivo displayed bands at 26 and 27 kDa, while bacteria cultured in vivo in the HMW bag possessed only the 27 kDa band. These bands may represent sialic acid. The significance of the changes observed in the bacterium's structure and antigenicity when cultured in vivo is discussed.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
Electron microscopic examination of Photobacterium damselae subsp. piscicida (I752) cultured in vivo and in vitro. Arrows indicate capsular material. (A) Bacteria grown in vivo in 300 kDa dialysis tubing (HMW bag). Bar = 0.1 µm. (B) Bacteria grown in vivo in 25 kDa dialysis tubing (LMW bag). Bar = 0.1 µm. (C) Bacteria grown in vitro in 300 kDa dialysis tubing (HMW bag). Bar = 0.2 µm.
Fig. 2
Fig. 2
Coomassie brilliant staining of SDS-PAGE of Photobacterium damselae subsp. piscicida and their extracellular products (ECP) after culturing in vivo and in vitro. Lane 1: molecular weight marker, Lane 2: bacteria maintained in vitro in 25 kDa dialysis tubing (LMW bag), Lane 3: concentrated ECP of bacteria shown in Lane 2, Lane 4: bacteria maintained in vitro in 300 kDa dialysis tubing (HMW bag), Lane 5: concentrated ECP of bacteria shown in Lane 4, Lane 6: bacteria maintained in vivo in 25 kDa dialysis tubing (LMW bag), Lane 7: concentrated ECP of bacteria shown in Lane 6, Lane 8: bacteria maintained in vivo in 300 kDa dialysis tubing (HMW bag), Lane 9: concentrated ECP of bacteria shown in Lane 8.
Fig. 3
Fig. 3
Western blot analysis with anti-Photobacterium damselae subsp. piscicida sea bass sera against Ph. d. subsp. piscicida (I752) and their extracellular products (ECP) after culture in vivo and in vitro. Lane 1: bacteria maintained in vitro in 25 kDa dialysis tubing (LMW bag), Lane 2: concentrated ECP of bacteria shown in Lane 1, Lane 3: bacteria maintained in vitro in 300 kDa dialysis tubing (HMW bag), Lane 4: concentrated ECP of bacteria shown in Lane 3, Lane 5: bacteria maintained in vivo in 25 kDa dialysis tubing (LMW bag), Lane 6: concentrated ECP of bacteria shown in Lane 5, Lane 7: bacteria maintained in vivo in 300 kDa dialysis tubing (HMW bag), Lane 8: concentrated ECP of bacteria shown in Lane 7.
Fig. 4
Fig. 4
Carbohydrate determination on Photobacterium damselae subsp. piscicida (I752) and in their extracellular products (ECP) after culture in vivo and in vitro. Lane 1: concentrated ECP of bacteria shown in Lane 2, Lane 2: bacteria maintained in vivo in 300 kDa dialysis tubing (HMW bag), Lane 3: concentrated ECP of bacteria shown in Lane 4, Lane 4: bacteria maintained in vivo in 25 kDa dialysis tubing (LMW bag), Lane 5: concentrated ECP of bacteria shown in Lane 6, Lane 6: bacteria maintained in vitro in 300 kDa dialysis tubing (HMW bag), Lane 7: concentrated ECP of bacteria shown in Lane 8, Lane 8: bacteria maintained in vitro in 25 kDa dialysis tubing (LMW bag).
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Arijo S, Balebona C, Martinez-Manzanares E, Moriñigo MA. Immune response of gilt-head seabream (Sparus aurata) to antigens from Photobacterium damselae subsp. piscicida. Fish Shellfish Immunol. 2004;16:65–70. - PubMed
    1. Arijo S, Borrego JJ, Zorrilla I, Balebona MC, Moriñigo MA. Role of the capsule of Photobacterium damsela subsp. piscicida in protection against phagocytosis and killing by gilt-head seabream (Sparus aurata, L) macrophages. Fish Shellfish Immunol. 1998;8:63–72.
    1. Bakopoulos V, Adams A, Richards RH. The effect of iron limitation growth conditions on the cell and extracellular components of the fish pathogen Pasteurella piscicida. J Fish Dis. 1997;20:297–305.
    1. Bakopoulos V, Hanif A, Poulos K, Galeotti M, Adams A, Dimitriadis GJ. The effect of in vivo growth on the cellular and extracellular components of the marine bacterial pathogen Photobacterium damsela subsp. piscicida. J Fish Dis. 2004;27:1–14. - PubMed
    1. Bakopoulos V, Pearson M, Volpatti D, Gousmani L, Adams A, Galeotti M, Dimitriadis GJ. Investigation of media formulations promoting differential antigen expression by Photobacterium damsela ssp. piscicida and recognition by sea bass, Dicentrarchus labrax (L.), immune sera. J Fish Dis. 2003;26:1–13. - PubMed

Publication types

MeSH terms