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. 2008 Jun 7:(21):2471-3.
doi: 10.1039/b801392f. Epub 2008 Mar 26.

TiO2 nanoparticles as a soft X-ray molecular probe

Jared M Ashcroft  1 , Weiwei Gu, Tierui Zhang, Steven M Hughes, Keith B Hartman, Cristina Hofmann, Antonios G Kanaras, David A Kilcoyne, Mark Le Gros, Yadong Yin, A Paul Alivisatos, Carolyn A Larabell
Affiliations

TiO2 nanoparticles as a soft X-ray molecular probe

Jared M Ashcroft et al. Chem Commun (Camb). .

Abstract

This communication reports the development of a TiO2-streptavidin nanoconjugate as a new biological label for X-ray bio-imaging applications; this new probe, used in conjunction with the nanogold probe, will make it possible to obtain quantitative, high-resolution information about the location of proteins using X-ray microscopy.

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Figures

Fig. 1
Fig. 1
TEM image of PAA coated TiO2 nanoparticles.
Fig. 2
Fig. 2
Fluorescent image of streptavidin-Alexa (a) and TiO2-streptavidin-Alexa (b) labelled microtubules. TEM image of unlabeled (c) and TiO2-streptavidin-Alexa labelled (d) microtubules. (e) Selected area electron diffraction (SAED) pattern of TiO2 (anatase) nanoparticles of the TiO2-labeled MT sample.
Fig. 3
Fig. 3
(a) STXM images TiO2 nanoparticles. (b) XANES spectrum of TiO2 nanoparticles indicated with the arrowhead in (a). (c) STXM images of TiO2 labeled mictrotubules. The absorption image shown was taken at 466 eV. The Ti map was obtained by subtracting the OD (optical density) image taken at 466 eV by the OD image taken at 450 eV. The C map was obtained by subtracting the OD image taken at 293 eV (above C/K-edge) by the OD image taken at 280 eV (below C/K-edge).
Scheme 1
Scheme 1
TiO2–streptavidin conjugation.
See this image and copyright information in PMC

References

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