Transposition of the maize activator element in transgenic rice plants

Abstract

Transposition of the maize Activator (Ac) element was observed in transgenic rice. After protoplast transformation, Ac excision from an interrupted hygromycin phosphotransferase gene was monitored by appearance of the hygromycin-resistant colonies. The frequency of Ac excision, based on the biological assay was up to 19%. Southern hybridization analysis indicated that at least one copy per genome of the hygromycin-resistance gene was reconstituted after Ac excision and that the transposed Ac element was reintegrated into the rice genome. Analysis of DNA sequences at 14 empty donor sites indicated that the Ac element was excised in rice in a similar manner as maize. The excision of an Ac mutant in which a 1.3 kbp Tn903 fragment was inserted at a unique BamHI site so as to disrupt binding of the putative transposase was not detected by DNA analysis. These results demonstrated that the maize Ac element might be used as an effective heterologous transposon for mutagenesis and gene tagging in rice, an important food crops.