[Immunoenzimatic detection of the Clostridium tetani bacterial toxin: an alternative to mice bioassays]
[Article in
Spanish]
Affiliations
- PMID: 18494295
Item in Clipboard
[Immunoenzimatic detection of the Clostridium tetani bacterial toxin: an alternative to mice bioassays]
[Article in
Spanish]
Rev Biol Trop.
2006 Jun.
Abstract
Cell-free extracts from 20 strains of Clostridium tetani isolated from soil samples, were tested for tetanus toxin production using an enzyme immunoassay. All the extracts were classified as positive for the toxin presence, and eight of them showed absorbance values corresponding to tetanus toxin concentrations between 3.2 and 88 ng/ml; thus, they fell within the linear absorbance range (0.135-0.317). All dilutions of toxin used to obtain the calibration curve (0.0071 to 1.1 ng) were lethal for mice.
Similar articles
-
Density and distribution of Clostridium tetani in the soil.Jpn J Exp Med. 1986 Apr;56(2):69-74. Jpn J Exp Med. 1986. PMID: 3525907
-
[Isolation and identification of Clostridium tetani in the soil of the Eastern Townships in the province of Quebec].Can J Microbiol. 1973 Dec;19(12):1513-8. Can J Microbiol. 1973. PMID: 4592357 French. No abstract available.
-
Isolation and some properties of nontoxigenic derivatives of a strain of Clostridium tetani.Biken J. 1977 Dec;20(3-4):105-15. Biken J. 1977. PMID: 352338
-
Current concepts in the management of Clostridium tetani infection.Expert Rev Anti Infect Ther. 2008 Jun;6(3):327-36. doi: 10.1586/14787210.6.3.327. Expert Rev Anti Infect Ther. 2008. PMID: 18588497 Review.
-
Vaccination against diphtheria and tetanus. Assays, antigens, adjuvants and application.APMIS Suppl. 1998;81:1-40. APMIS Suppl. 1998. PMID: 9810281 Review. No abstract available.