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Randomized Controlled Trial
. 2008 Jun;5(2):195-203.
doi: 10.1111/j.1742-481X.2007.00367.x.

Effect of oxidised regenerated cellulose/collagen matrix on proteases in wound exudate of patients with chronic venous ulceration

Ralf Smeets  1 , Dietmar Ulrich, Frank Unglaub, Michael Wöltje, Norbert Pallua
Affiliations
Randomized Controlled Trial

Effect of oxidised regenerated cellulose/collagen matrix on proteases in wound exudate of patients with chronic venous ulceration

Ralf Smeets et al. Int Wound J. 2008 Jun.

Abstract

Oxidised regenerated cellulose/collagen matrix (ORC/collagen matrix) modifies wound microenvironments by binding and inactivating excess levels of proteases such as elastase, plasmin and gelatinases in wound exudates. To compare levels of the gelatinases matrix metalloproteinase 2 (MMP-2), elastase and plasmin in wound exudates collected from chronic venous insufficiency patients with venous leg ulcers treated with either an ORC/collagen matrix or a standard control therapy. During a 12-week treatment period, wound exudate samples were obtained from a control group of 10 patients treated with a hydrocolloid dressing and a treatment group of 17 patients treated with a combination of ORC/collagen matrix and hydrocolloid dressing. On admission and days 5, 14 and every subsequent 14th day, ulcers were photographed to determine healing rate and changes in ulcer appearance, and MMP-2 concentration and the gelatinase, elastase and plasmin activities were analysed from wound exudates. The patients treated with ORC/collagen matrix showed a significant decrease in elastase, plasmin and gelastinase activity as compared with the control group, with no significant difference in the MMP-2 concentrations between the two groups. The results show a significant and immediate reduction in protease activity in wound exudates from venous leg ulcers treated with ORC/collagen.

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Figures

Figure 1
Figure 1
MMP‐2 (pg/ml/mg protein) in wound exudates of patients with chronic venous insufficiency.
Figure 2
Figure 2
Gelatinase activity (%/ml/mg protein) in wound exudates of patients with chronic venous insufficiency. #P < 0·05 versus day 0.
Figure 3
Figure 3
Elastase activity (μU/ml/mg protein) in wound exudates of patients with chronic venous insufficiency. *P < 0·05 versus oxidised regenerated cellulose/collagen matrix and #P < 0·05 versus day 0.
Figure 4
Figure 4
Plasmin activity (μU/ml/mg protein) in wound exudates of patients with chronic venous insufficiency.
See this image and copyright information in PMC

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