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. 2008 May;8(2):110-5.
doi: 10.17305/bjbms.2008.2962.

Daily fluctuation of cortisol in the saliva and serum of healthy persons

Nevzeta Ljubijankić  1 , Ranka Popović-Javorić, Sabiha Sćeta, Aida Sapcanin, Ismet Tahirović, Emin Sofić
Affiliations

Daily fluctuation of cortisol in the saliva and serum of healthy persons

Nevzeta Ljubijankić et al. Bosn J Basic Med Sci. 2008 May.

Abstract

The objective of this study was to evaluate cortisol in the saliva and serum of healthy persons and its daily fluctuations by using immunochemical method on a autoanalyzer. Biological samples: Serum from 14 healthy persons and saliva from 18 healthy persons were taken two times at 8 a.m. and at 4 p.m. Immunochemical assay: The principle of this method is the competitive binding of cortisol present in the analyzed sample and cortisol marked with peroxides on binding parts with specific antibodies.

Statistical analysis: Student t-test. Cortisol in saliva in the morning: 21,2 +/- 16,2 nmol/l and in the afternoon 12,7 +/- 8,1 nmol/l. Cortisol in serum in the morning: 459, 6 +/- 235,2 nmol/l , and in the afternoon 340,5 +/- 207,5 nmol/l. The concentrations of cortisol in saliva are lower than in serum. Cortisol in the serum in the morning is about twenty times higher than cortisol in the saliva at the same time. Cortisol in the serum at afternoon is about twenty-seven times higher than cortisol in the saliva. Individual variabilities of cortisol in the saliva and serum were found during the day.

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Figures

FIGURE 1
FIGURE 1
Cholesterol as predecessor of steroid hormons
FIGURE 2
FIGURE 2
11 p, 17a, 21 - trihydroxypregnan - 4 - en - 3,20 -dion (Cortisol)
GRAPH 1
GRAPH 1
Concentration of Cortisol in saliva of healthy persons in the morning and afternoon
GRAPH 2
GRAPH 2
Concentration of Cortisol in serum of 14 healthy persons in the morning and afternoon
GRAPH 3
GRAPH 3
Comparison of Cortisol concentration in saliva and serum in the morning and afternoon
See this image and copyright information in PMC

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