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. 1991 Feb 25;19(4):747-50.
doi: 10.1093/nar/19.4.747.

Modified deoxyoligonucleotides stable to exonuclease degradation in serum

J P Shaw  1 , K Kent, J Bird, J Fishback, B Froehler
Affiliations
Free PMC article

Modified deoxyoligonucleotides stable to exonuclease degradation in serum

J P Shaw et al. Nucleic Acids Res. .
Free PMC article

Abstract

Unmodified deoxyoligonucleotides are rapidly degraded in serum-containing medium. Utilizing internally labelled deoxyoligonucleotides the deoxyribonuclease profile for fetal calf serum and human serum was determined. It was found that the predominate nuclease activity in both systems was 3' exonuclease. Deoxyoligonucleotides are protected from exonuclease degradation in sera and cell media by simple terminal modifications that maintain high binding activity for the complementary DNA sequence.

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