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Comparative Study
. 2008 Jul;74(14):4539-42.
doi: 10.1128/AEM.00284-08. Epub 2008 May 23.

Analysis of vaginal lactobacilli from healthy and infected Brazilian women

Rafael C R Martinez  1 , Sílvio A Franceschini, Maristela C Patta, Silvana M Quintana, Alvaro C Nunes, João L S Moreira, Kingsley C Anukam, Gregor Reid, Elaine C P De Martinis
Affiliations
Comparative Study

Analysis of vaginal lactobacilli from healthy and infected Brazilian women

Rafael C R Martinez et al. Appl Environ Microbiol. 2008 Jul.

Abstract

Culture-dependent PCR-amplified rRNA gene restriction analysis and culture-independent (PCR-denaturing gradient gel electrophoresis) methodologies were used to examine vaginal lactobacilli from Brazilian women who were healthy or had been diagnosed with vulvovaginal candidiasis (VVC) or bacterial vaginosis. Only Lactobacillus crispatus was detected accordingly by both methods, and H(2)O(2)-producing lactobacilli were not associated with protection against VVC.

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Figures

FIG. 1.
FIG. 1.
Prevalence of vaginal lactobacilli obtained from three groups of Brazilian patients comprising 64 healthy women (A), 68 women diagnosed with VVC (B), and 64 women diagnosed with BV (C), according to results assessed by PCR-ARDRA and PCR-DGGE.
See this image and copyright information in PMC

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