Enzymatic excision of radiation-induced lesions from DNA model compounds
Affiliations
- PMID: 1850532
Item in Clipboard
Enzymatic excision of radiation-induced lesions from DNA model compounds
Radiat Res.
1991 Apr.
Abstract
Dinucleoside monophosphates in which the 5' nucleoside contained a radiation-modified base were tested as substrates to bovine spleen phosphodiesterase (SPD) and snake venom phosphodiesterase. The radiation-modified bases included thymine glycols, 5-hydroxymethyluracil, 8-hydroxyguanine, and a formamido remnant of thymine. The lesions had widely different effects on diesterase action, varying from little inhibition, as in the case of digestion of dT*pA by SPD, where T* is the hydroxymethyluracil modification, to severe inhibition, as in the case of digestion of dG*pC by SPD, where G* is the 8-hydroxyguanine modification.
Similar articles
-
32P-postlabeling detection of radiation-induced DNA damage: identification and estimation of thymine glycols and phosphoglycolate termini.Biochemistry. 1991 Jan 29;30(4):1091-7. doi: 10.1021/bi00218a031. Biochemistry. 1991. PMID: 1846559
-
Double-base lesions are produced in DNA by free radicals.Arch Biochem Biophys. 2000 Mar 1;375(1):119-23. doi: 10.1006/abbi.1999.1640. Arch Biochem Biophys. 2000. PMID: 10683256
-
Evaluation of different types of end-capping modifications on the stability of oligonucleotides toward 3'- and 5'-exonucleases.Nucleosides Nucleotides. 1999 Sep;18(9):2051-69. doi: 10.1080/07328319908044864. Nucleosides Nucleotides. 1999. PMID: 10549151
-
Postlabelling methods for the detection of apurinic sites and radiation-induced DNA damage.IARC Sci Publ. 1993;(124):95-103. IARC Sci Publ. 1993. PMID: 8225514 Review.
-
Enzymatic processing of radiation-induced free radical damage in DNA.Radiat Res. 1998 Nov;150(5 Suppl):S60-79. Radiat Res. 1998. PMID: 9806610 Review.
Cited by
-
Mechanisms of induction and repair of DNA double-strand breaks by ionizing radiation: some contradictions.Radiat Environ Biophys. 1994;33(1):45-61. doi: 10.1007/BF01255273. Radiat Environ Biophys. 1994. PMID: 8202592 Review.
-
Endogenous oxidative damage of deoxycytidine in DNA.Proc Natl Acad Sci U S A. 1992 Apr 15;89(8):3380-4. doi: 10.1073/pnas.89.8.3380. Proc Natl Acad Sci U S A. 1992. PMID: 1565630 Free PMC article.
-
Endonuclease and Exonuclease Activities on Oligodeoxynucleotides Containing Spiroiminodihydantoin Depend on the Sequence Context and the Lesion Stereochemistry.New J Chem. 2013 Nov 1;37(11):3440-3449. doi: 10.1039/C3NJ00418J. New J Chem. 2013. PMID: 24563606 Free PMC article.
-
Photooxidation of d(TpG) by riboflavin and methylene blue. Isolation and characterization of thymidylyl-(3',5')-2-amino-5-[(2-deoxy-beta-D- erythro-pentofuranosyl)amino]-4H-imidazol-4-one and its primary decomposition product thymidylyl-(3',5')-2,2-diamino-4-[(2-deoxy-beta-D- erythro-pentofuranosyl)amino]-5(2H)-oxazolone.Nucleic Acids Res. 1995 Oct 11;23(19):3954-61. doi: 10.1093/nar/23.19.3954. Nucleic Acids Res. 1995. PMID: 7479042 Free PMC article.
-
Influence of nucleic acid base aromaticity on substrate reactivity with enzymes acting on single-stranded DNA.Nucleic Acids Res. 1993 Feb 11;21(3):621-6. doi: 10.1093/nar/21.3.621. Nucleic Acids Res. 1993. PMID: 8382802 Free PMC article.