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. 2008 May 28:6:8.
doi: 10.1186/1546-0096-6-8.

Elevated serum levels of soluble CD154 in children with juvenile idiopathic arthritis

Sampath Prahalad  1 , Thomas B Martins, Anne E Tebo, April Whiting, Bronte Clifford, Andrew S Zeft, Bernadette McNally, John F Bohnsack, Harry R Hill
Affiliations

Elevated serum levels of soluble CD154 in children with juvenile idiopathic arthritis

Sampath Prahalad et al. Pediatr Rheumatol Online J. .

Abstract

Objective: Cytokines play important roles in mediating inflammation in autoimmunity. Several cytokines are elevated in serum and synovial fluid samples from children with Juvenile Idiopathic Arthritis (JIA). Soluble CD154 (sCD154) is elevated in other autoimmune disorders, but has not been characterized in JIA. Our objectives were to determine if sCD154 is elevated in JIA, and to examine correlations between sCD154 and other inflammatory cytokines.

Methods: Serum from 77 children with JIA and 81 pediatric controls was analyzed for interleukin (IL)1beta, IL2, IL4, IL5, IL6, IL8, IL10, IL12, IL13, sCD154, interferon-gamma (IFNgamma), soluble IL2 receptor (sIL2R), and tumor necrosis factor-alpha (TNFalpha), using the Luminex Multi-Analyte Profiling system. Differences in levels of cytokines between cases and controls were analyzed. Logistic regression was also performed.

Results: sCD154 was significantly elevated in cases compared to controls (p < 0.0001). IL1beta, IL5, IL6, IL8, IL13, IFNgamma, sIL2R, and TNFalpha were also significantly elevated in JIA. Levels of sCD154 were highly correlated with IL1beta, IL6, IL8, and TNFalpha (p < 0.0001). Logistic regression analysis suggested that IL6 (odds ratio (OR): 1.4, p < 0.0001), sCD154 (OR: 1.1, p < 0.0001), and TNFalpha (OR: 1.1, p < 0.005) were positively associated with JIA, while IL10 (OR: 0.5, p < 0.002) was protective. sCD154 was elevated in all JIA subtypes, with highest levels among more severe subtypes. IL1beta, IL6, IL8, sIL2R and TNFalpha were also elevated in several JIA subtypes.

Conclusion: Serum levels of sCD154, IL1beta, IL6, IL8, sIL2R and TNFalpha are elevated in most JIA subtypes, suggesting a major role for sCD154, and these cytokines and cytokine receptors in the pathogenesis of JIA.

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Figures

Figure 1
Figure 1
sCD154 Levels among controls and children with different JIA subtypes. Levels of sCD154 in different JIA subtypes. The boundaries of the boxes correspond to the 25th and 75th percentile. The solid horizontal line within the box denotes the median value and the dashed lines represent the mean values. The Whiskers above and below the boxes show the 90th and 10th percentiles respectively. Four subjects had levels greater than 1000 pg/ml.
See this image and copyright information in PMC

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