doi: 10.1016/0378-1135(91)90040-m.
Enzyme-linked immunosorbent assay for the detection of canine coronavirus and its antibody in dogs
Affiliations
- PMID: 1850890
- PMCID: PMC7117253
- DOI: 10.1016/0378-1135(91)90040-m
Item in Clipboard
Enzyme-linked immunosorbent assay for the detection of canine coronavirus and its antibody in dogs
Vet Microbiol.
1991 Jan.
Abstract
Two methods of enzyme-linked immunosorbent assay (ELISA) were developed for the diagnosis of canine coronavirus (CCV) infection in dogs. One ELISA, in which CCV-infected CRFK cell lysate is used as antigen, is for the detection and titration of antibody against CCV, and the other ELISA uses the double antibody sandwich method for the detection of CCV antigen. The first ELISA procedure demonstrated antibody responses in dogs inoculated with CCV, as did the virus neutralization test; the second ELISA detected specific CCV antigen in feces and organ homogenates of inoculated dogs.
Similar articles
-
An enzyme-linked immunosorbent assay using canine coronavirus-infected CRFK cells as antigen for detection of anti-coronavirus antibody in cat.Comp Immunol Microbiol Infect Dis. 1989;12(4):139-46. doi: 10.1016/0147-9571(89)90062-3. Comp Immunol Microbiol Infect Dis. 1989. PMID: 2558837 Free PMC article.
-
The use of enzyme-linked immunosorbent assay systems for serology and antigen detection in parvovirus, coronavirus and rotavirus infections in dogs in The Netherlands.Vet Microbiol. 1991 Jan;26(1-2):25-40. doi: 10.1016/0378-1135(91)90039-i. Vet Microbiol. 1991. PMID: 1850889 Free PMC article.
-
Characterization of monoclonal and polyclonal antibodies to bovine enteric coronavirus: establishment of an efficient ELISA for antigen detection in feces.Vet Microbiol. 1989 Jun;20(2):111-22. doi: 10.1016/0378-1135(89)90034-5. Vet Microbiol. 1989. PMID: 2549679 Free PMC article.
-
New enteric viruses in the dog.Adv Vet Sci Comp Med. 1981;25:1-37. Adv Vet Sci Comp Med. 1981. PMID: 6275684 Review. No abstract available.
-
Canine viral enteritis. Recent developments.Mod Vet Pract. 1979 May;60(5):375-80. Mod Vet Pract. 1979. PMID: 224304 Review.
Cited by
-
Identification of canine coronavirus strains from feces by S gene nested PCR and molecular characterization of a new Australian isolate.J Clin Microbiol. 2001 Mar;39(3):1036-41. doi: 10.1128/JCM.39.3.1036-1041.2001. J Clin Microbiol. 2001. PMID: 11230424 Free PMC article.
-
Canine coronavirus in Australian dogs.Aust Vet J. 2001 Feb;79(2):116-9. doi: 10.1111/j.1751-0813.2001.tb10718.x. Aust Vet J. 2001. PMID: 11256282 Free PMC article.
-
Antibody testing against canine coronavirus by immunoperoxidase plaque staining.Vet Res Commun. 2001 May;25(4):327-36. doi: 10.1023/a:1010634810315. Vet Res Commun. 2001. PMID: 11432433 Free PMC article.
-
Prevalence of canine coronavirus antibodies by an enzyme-linked immunosorbent assay in dogs in the south of Italy.J Virol Methods. 2002 Apr;102(1-2):67-71. doi: 10.1016/s0166-0934(01)00450-5. J Virol Methods. 2002. PMID: 11879694 Free PMC article.
References
-
- Appel M.J.G., Cooper B.J., Greisen H., Scott F., Carmichael L.E. Canine viral enteritis. I. Status report on corona- and parvo-like viral enteritides. Cornell Vet. 1979;69:123–133. - PubMed
-
- Appel M., Meunier P., Pollock R., Greisen H., Carmichael L., Glickman L. Canine viral enteritis. Canine Pract. 1980;7:22–36.
-
- Binn L.N., Lazar E.C., Keenan K.P., Huxsoll D.L., Marchwicki R.H., Strano A.J. Recovery and characterization of a coronavirus from military dogs with diarrhea. Proc. 78th Annu. Meet. U.S. Animal Health Association; 1974; 1975. pp. 359–366. - PubMed
-
- Carmichael L.E., Binn L.N. New enteric viruses in the dog. Adv. Vet. Sci. Comp. Med. 1981;25:1–37. - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
