Characterization of Leishmania (Leishmania) amazonensis promastigotes resistant to pentamidine
- PMID: 18511047
- DOI: 10.1016/j.exppara.2008.03.018
Characterization of Leishmania (Leishmania) amazonensis promastigotes resistant to pentamidine
Abstract
Pentamidine is a second-line agent used in the treatment of leishmaniasis and its mode of action and mechanism of resistance is not well understood. It was previously demonstrated that transfection of promastigotes and amastigotes with the ABC transporter PRP1 gene confers resistance to pentamidine. To further clarify this point, we generated Leishmania amazonensis mutants resistant to pentamidine. Our results indicated that this ABC transporter is not associated with pentamidine resistance in lines generated by drug pressure through amplification or overexpression mechanisms of PRP1 gene.
Similar articles
-
Role of the ABC transporter PRP1 (ABCC7) in pentamidine resistance in Leishmania amastigotes.Antimicrob Agents Chemother. 2007 Aug;51(8):3030-2. doi: 10.1128/AAC.00404-07. Epub 2007 Apr 23. Antimicrob Agents Chemother. 2007. PMID: 17452480 Free PMC article.
-
Functional genetic identification of PRP1, an ABC transporter superfamily member conferring pentamidine resistance in Leishmania major.Mol Biochem Parasitol. 2003 Aug 31;130(2):83-90. doi: 10.1016/s0166-6851(03)00162-2. Mol Biochem Parasitol. 2003. PMID: 12946844
-
Intracellular location of the ABC transporter PRP1 related to pentamidine resistance in Leishmania major.Mol Biochem Parasitol. 2006 Dec;150(2):378-83. doi: 10.1016/j.molbiopara.2006.08.013. Epub 2006 Sep 20. Mol Biochem Parasitol. 2006. PMID: 17030436 No abstract available.
-
Pentamidine uptake and resistance in pathogenic protozoa: past, present and future.Trends Parasitol. 2003 May;19(5):232-9. doi: 10.1016/s1471-4922(03)00069-2. Trends Parasitol. 2003. PMID: 12763430 Review.
-
Leishmaniasis: efflux pumps and chemoresistance.Int J Antimicrob Agents. 2003 Sep;22(3):352-7. doi: 10.1016/s0924-8579(03)00211-5. Int J Antimicrob Agents. 2003. PMID: 13678842 Review.
Cited by
-
New Approaches to Overcome Transport Related Drug Resistance in Trypanosomatid Parasites.Front Pharmacol. 2016 Sep 28;7:351. doi: 10.3389/fphar.2016.00351. eCollection 2016. Front Pharmacol. 2016. PMID: 27733833 Free PMC article. Review.
-
ABC transporter inhibition by beauvericin partially overcomes drug resistance in Leishmania tropica.Antimicrob Agents Chemother. 2024 May 2;68(5):e0136823. doi: 10.1128/aac.01368-23. Epub 2024 Apr 4. Antimicrob Agents Chemother. 2024. PMID: 38572959 Free PMC article.
-
Antimony resistance in leishmania, focusing on experimental research.J Trop Med. 2011;2011:695382. doi: 10.1155/2011/695382. Epub 2011 Nov 17. J Trop Med. 2011. PMID: 22174724 Free PMC article.
-
Repurposing Lansoprazole and Posaconazole to treat leishmaniasis: Integration of in vitro testing, pharmacological corroboration, and mechanisms of action.J Food Drug Anal. 2022 Mar 15;30(1):128-149. doi: 10.38212/2224-6614.3394. J Food Drug Anal. 2022. PMID: 35647721 Free PMC article.
-
Comparative analysis of the omics technologies used to study antimonial, amphotericin B, and pentamidine resistance in leishmania.J Parasitol Res. 2014;2014:726328. doi: 10.1155/2014/726328. Epub 2014 May 12. J Parasitol Res. 2014. PMID: 24900912 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources