[Metabolism of nicotinic acid in plant cell suspension cultures, III: Formation and metabolism of trigonelline (author's transl)]

Abstract

Cell suspension cultures of Phaseolus aureus, Glycinemax., Cicer arietinum and Chenopodium rubrum convert nicotinic acid and nicotinamide into N-methyl nicotinic acid (trigonelline). Application of [carboxyl-14C]- and [N-methyl-14C]nicotinic acid to cell cultures demonstrated that 1) the nicotinic acid moiety of trigonelline is funnelled into the pyridine nucleotide cycle, 2) trigonelline is demethylated partly oxidatively, but predominantly non-oxidatively, transferring the methyl carbon atom to still unknown acceptors, and 3) uptake of trigonelline by mung bean cell cultures is accompanied by demethylation and instantaneous remethylation reactions. Cell suspension cultures of parsley (Petroselinum hortense Hoffm.) show uptake but no metabolism of trigonelline. The data are compared with trigonelline metabolism in intact plants.