Comparative analysis of divergent and convergent gene pairs and their expression patterns in rice, Arabidopsis, and populus

Abstract

Comparative analysis of the organization and expression patterns of divergent and convergent gene pairs in multiple plant genomes can identify patterns that are shared by more than one species or are unique to a particular species. Here, we study the coexpression and interspecies conservation of divergent and convergent gene pairs in three plant species: rice (Oryza sativa), Arabidopsis (Arabidopsis thaliana), and black cottonwood (Populus trichocarpa). Strongly correlated expression levels between divergent and convergent genes were found to be quite common in all three species, and the frequency of strong correlation appears to be independent of intergenic distance. Conservation of divergent or convergent arrangement among these species appears to be quite rare. However, conserved arrangement is significantly more frequent when the genes display strongly correlated expression levels or have one or more Gene Ontology (GO) classes in common. A correlation between intergenic distance in divergent and convergent gene pairs and shared GO classes was observed, in varying degrees, in rice and Populus but not in Arabidopsis. Furthermore, multiple GO classes were either overrepresented or underrepresented in Arabidopsis and Populus gene pairs, while only two GO classes were underrepresented in rice divergent gene pairs. Three cis-regulatory elements common to both Arabidopsis and rice were overrepresented in the intergenic regions of strongly correlated divergent gene pairs compared to those of noncorrelated pairs. Our results suggest that shared as well as unique mechanisms operate in shaping the organization and function of divergent and convergent gene pairs in different plant species.

Figure 1.

A, Fractions of divergent gene pairs with matching EST or cDNA sequences for both genes in rice, Arabidopsis, and Populus. “Total” represents the entire population of divergent gene pairs in each species, while <1 kb, <500 bp, and <250 bp each represent a subset of the population with these maximum distances between the genes in a pair. B, Fractions of convergent gene pairs with matching EST or cDNA sequences for both genes in rice, Arabidopsis, and Populus. “Total” represents the entire population of convergent gene pairs in each species, while <1 kb, <500 bp, and <250 bp each represent a subset of the population with these maximum distances between the genes in a pair.