Catabolism of 3- and 4-hydroxyphenylacetic acid by Klebsiella pneumoniae
- PMID: 1851804
- DOI: 10.1099/00221287-137-3-621
Catabolism of 3- and 4-hydroxyphenylacetic acid by Klebsiella pneumoniae
Abstract
Klebsiella pneumoniae catabolizes both 4-hydroxyphenylacetic acid and 3-hydroxyphenylacetic acid via meta-cleavage of 3,4-dihydroxyphenylacetic acid, ultimately yielding pyruvate and succinate. The organism can synthesize two hydroxylases catalysing 3,4-dihydroxyphenylacetic acid formation, which differ in substrate specificity, cofactor requirement, kinetics and regulation. Five enzymes sequentially involved in the catabolism of 3,4-dihydroxyphenylacetic acid are encoded on a 7 kbp fragment of the K. pneumoniae chromosome that has been isolated in a recombinant plasmid.
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