Simultaneous detection and typing of genital human papillomavirus DNA using the polymerase chain reaction
- PMID: 1851805
- DOI: 10.1099/0022-1317-72-5-1039
Simultaneous detection and typing of genital human papillomavirus DNA using the polymerase chain reaction
Abstract
A simple method has been developed for detecting a broad range of genital human papillomavirus (HPV) types using the polymerase chain reaction (PCR). We utilized two consensus sequence primer pairs within the E6 and E7 open reading frames to amplify HPV DNA; malignant HPV DNA (from HPV-16, -18, -31, -33, -52b and -58) was amplified using the pU-1M/pU-2R primer pair whereas benign HPV DNA (from HPV-6 and -11) was amplified using the pU-31B/pU-2R primer pair. Identification of the amplification product was confirmed by restriction enzyme digestion. In this study, a pU-1M/pU-2R-mediated PCR was successfully applied to 39 cervical carcinoma specimens; HPV-16 was detected in 19 cases, HPV-18 in five cases, HPV-31 in two cases, HPV-33 in two cases, HPV-52b in one case, HPV-58 in three cases, and an unknown type(s) was detected in four cases. Overall, the prevalence of HPV was 84.6%. The results indicate that this detection system is useful for the detection of HPVs not only of known types but also of new types.
Similar articles
-
Evaluation of human papillomavirus-consensus primers for HPV detection by the polymerase chain reaction.Mol Cell Probes. 1999 Feb;13(1):9-21. doi: 10.1006/mcpr.1998.0203. Mol Cell Probes. 1999. PMID: 10024428
-
Detection of human papillomavirus in cervical cell specimens by hybrid capture and PCR with different primers.Acta Biochim Pol. 2006;53(3):603-7. Epub 2006 Oct 1. Acta Biochim Pol. 2006. PMID: 17019439
-
Detection and typing of multiple genital human papillomaviruses by DNA amplification with consensus primers.Jpn J Cancer Res. 1991 May;82(5):524-31. doi: 10.1111/j.1349-7006.1991.tb01882.x. Jpn J Cancer Res. 1991. PMID: 1648051 Free PMC article.
-
[Amplification and typing of human papillomavirus (HPV) DNA from female genitalia by polymerase chain reaction using a consensus primer].Nihon Rinsho. 1992 Jul;50 Suppl:199-202. Nihon Rinsho. 1992. PMID: 1328712 Review. Japanese. No abstract available.
-
Human Papillomavirus Genome based Detection and Typing: A Holistic Molecular Approach.Curr Mol Med. 2019;19(4):237-246. doi: 10.2174/1566524019666190405120441. Curr Mol Med. 2019. PMID: 30950352 Review.
Cited by
-
Sequence variation of human papillomavirus type 16 E7 in preinvasive and invasive cervical neoplasias.Virus Genes. 1994 Sep;9(1):85-92. doi: 10.1007/BF01703438. Virus Genes. 1994. PMID: 7871765
-
Intermethod variation in detection of human papillomavirus DNA in cervical smears.J Clin Microbiol. 1995 Oct;33(10):2631-6. doi: 10.1128/jcm.33.10.2631-2636.1995. J Clin Microbiol. 1995. PMID: 8567896 Free PMC article. Clinical Trial.
-
Clinicopathological characteristics of esophageal squamous papillomas in Japanese patients--with comparison of findings from Western countries.Acta Histochem Cytochem. 2006 Mar 2;39(1):23-30. doi: 10.1267/ahc.05052. Acta Histochem Cytochem. 2006. PMID: 17460769 Free PMC article.
-
Absence of human papillomavirus genomic sequences detected by the polymerase chain reaction in oesophageal and gastric carcinomas in Japan.Mol Pathol. 1997 Apr;50(2):101-4. doi: 10.1136/mp.50.2.101. Mol Pathol. 1997. PMID: 9231159 Free PMC article.
-
Human papillomavirus and p53 mutations in head and neck squamous cell carcinoma among Japanese population.Cancer Sci. 2014 Apr;105(4):409-17. doi: 10.1111/cas.12369. Epub 2014 Mar 30. Cancer Sci. 2014. PMID: 24521534 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources