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. 2008 Jun;147(2):446-55.
doi: 10.1104/pp.108.118828.

Bacterial RNA chaperones confer abiotic stress tolerance in plants and improved grain yield in maize under water-limited conditions

Paolo Castiglioni  1 , Dave Warner, Robert J Bensen, Don C Anstrom, Jay Harrison, Martin Stoecker, Mark Abad, Ganesh Kumar, Sara Salvador, Robert D'Ordine, Santiago Navarro, Stephanie Back, Mary Fernandes, Jayaprakash Targolli, Santanu Dasgupta, Christopher Bonin, Michael H Luethy, Jacqueline E Heard
Affiliations

Bacterial RNA chaperones confer abiotic stress tolerance in plants and improved grain yield in maize under water-limited conditions

Paolo Castiglioni et al. Plant Physiol. 2008 Jun.
No abstract available

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Figures

Figure 1.
Figure 1.
Transgenic Arabidopsis seedlings demonstrate improved growth under constant light conditions at 8°C for 6 weeks. Transgenic Arabidopsis seedlings expressing CspA or CspB are displayed on the right and exhibit more growth under these conditions relative to nontransgenic controls. A, CspA-negative control. B, CspA-positive transgenic. C, CspB-negative control. D, CspB-positive transgenic. The positive growth effect was only observed under the chilling stress conditions and not at 25°C (data not shown). Experimental details are described in Supplemental Materials and Methods S1.
Figure 2.
Figure 2.
A single transgenic CspB event demonstrates yield improvements across multiple years of testing under water-stressed environments. Performance of CspB-Zm event 1 (black diamonds) and the appropriate control (white circles) were plotted against the location average yields across the past three seasons of testing under managed stress environments. x axis, Average yield (tons/hectare) at each location; y axis, mean yield (tons/hectare) of the entries, by location. Experimental details are described in Supplemental Materials and Methods S1.
Figure 3.
Figure 3.
Yield results from Midwest evaluations under water-deficit conditions. Three different hybrids carrying a single transgenic event expressing CspB were evaluated in yield trials across the western dryland market. Yield results were averaged across locations that experienced water-deficit stress during the late vegetative or grain fill periods of the season. Experimental details are described in Supplemental Materials and Methods S1.
Figure 4.
Figure 4.
Single-stranded DNA probe opening ability for B. subtilis CspB and the CspB_F30R mutant. Probe opening assay for BsCspB, BsCspBF30R, and controls. Negative control contained all elements that Csp samples contained except protein. The average of three independent replications is represented (±sd). The y axis is labeled as the average relative fluorescence units (RFU). Experimental details are described in Supplemental Materials and Methods S1.
Figure 5.
Figure 5.
CspA (A), CspB (B), and cotton CSP-like 1 (C) GFP fusion proteins accumulate in the cytoplasm, nucleus, and nucleolus when expressed in maize protoplasts. D, Results obtained with an AtCBF3 transcription factor:GFP fusion protein that localizes exclusively to the nucleus. Experimental details are described in Supplemental Materials and Methods S1.
See this image and copyright information in PMC

References

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