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. 2008 Jul 2;9(10):1576-8.
doi: 10.1002/cbic.200800039.

Tetrameric beta(3)-peptide bundles

Jessica L Goodman  1 , Matthew A Molski, Jade Qiu, Alanna Schepartz
Affiliations

Tetrameric beta(3)-peptide bundles

Jessica L Goodman et al. Chembiochem. .
No abstract available

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Figures

Figure 1
Figure 1
(A) Helical net representation of Zwit-1F, Acid-1Y, Zwit-VY and Acid-VY. (B, C) Zwit-VY and Acid-VY self-association monitored by analytical ultracentrifugation and fit to monomer-n-mer equilibria. Samples were prepared in 10 mM NaH2PO4, 200 mM NaCl (pH 7.1) and centrifuged to equilibrium at 25 °C at the indicated speed. The experimental data points are shown as open circles; lines indicate fits to the indicated monomer-n models.
Figure 2
Figure 2
Zwit-VY self-association monitored by circular dichroism spectroscopy (CD). (A) Plot of MRE209 as a function of [Zwit-VY]monomer fit to a monomer-tetramer equilibrium. (B) Plot of δMRE209•δT−1 for the concentrations of Zwit-VY shown.
Figure 3
Figure 3
Kinetic stability of the Zwit-VY tetramer as determined by hydrogen/deuterium exchange analysis. (A) 500 MHz 1H NMR spectra of 0.6 mM Zwit-VY acquired at the indicated times after a lyophilized Zwit-VY sample was reconstituted in phosphate-buffered D2O. (B) Peak heights of the indicated resonances (normalized to a peak at 6.8 ppm, *) fit to exponential decays as described in Supplementary Information.
See this image and copyright information in PMC

References

    1. Goodman CM, Choi S, Shandler S, DeGrado WF. Nat. Chem. Biol. 2007;3:252–262. - PMC - PubMed
    1. Bautista AD, Craig CJ, Harker EA, Schepartz A. Curr. Opin. Chem. Biol. 2007;11:685–692. - PMC - PubMed
    1. Qiu JX, Petersson EJ, Matthews EE, Schepartz A. J. Am. Chem. Soc. 2006;128:11338–11339. - PMC - PubMed
    1. Daniels DS, Petersson EJ, Qiu JX, Schepartz A. J. Am. Chem. Soc. 2007;129:1532–1533. - PMC - PubMed
    1. Petersson EJ, Craig CJ, Daniels DS, Qiu JX, Schepartz A. J. Am. Chem. Soc. 2007;129:5344–5345. - PMC - PubMed

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