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. 1991 Aug;183(2):782-5.
doi: 10.1016/0042-6822(91)91011-5.

In vitro membrane binding of the translation products of the carlavirus 7-kDa protein genes

S Y Morozov  1 , N A Miroshnichenko, A G Solovyev, D A Zelenina, O N Fedorkin, L I Lukasheva, S A Grachev, B K Chernov
Affiliations

In vitro membrane binding of the translation products of the carlavirus 7-kDa protein genes

S Y Morozov et al. Virology. 1991 Aug.

Abstract

Two double-stranded DNA copies of the genes potentially coding for the 7-kDa proteins of potato virus M (PVM) and potato virus S (PVS) were synthesized and cloned into T7 transcription vectors. Cell-free translation of the corresponding monocistronic transcripts yielded in both cases a single protein of approximately 7-8 kDa that contains a highly hydrophobic N-terminal segment. To analyze their membrane-binding potential, both proteins were synthesized in the membrane-enriched Krebs-2 extract. It was found that the smooth membrane fraction was enriched in the carlavirus 7-kDa proteins. The primary and predicted secondary structures of their N-terminal hydrophobic segments suggest that the latter can function as signals for translocation into the rough endoplasmic reticulum.

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