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Comment
. 2008 Jun;26(6):650-1.
doi: 10.1038/nbt0608-650.

Targeted mutagenesis in zebrafish

Ian G Woods  1 , Alexander F Schier
Affiliations
Comment

Targeted mutagenesis in zebrafish

Ian G Woods et al. Nat Biotechnol. 2008 Jun.
No abstract available

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Figures

Figure 1
Figure 1. Targeted mutagenesis of zebrafish genes with zinc-finger nucleases (ZFNs)
Two ZFNs that target the locus of interest are generated through rational design and screening. mRNAs encoding the ZFNs are injected into zebrafish embryos at the one-cell stage. After cleavage of the target sequence, the endogenous repair machinery can religate the DNA through nonhomologous end joining, which often adds or deletes nucleotides at the cleavage site.
See this image and copyright information in PMC

Comment on

References

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