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. 2008 Sep 1;380(1):152-4.
doi: 10.1016/j.ab.2008.05.014. Epub 2008 May 17.

Determining the concentration and the absorptivity factor at 260 nm in sodium dodecyl sulfate of the adenovirus reference material using analytical ultracentrifugation

Steven A Berkowitz  1
Affiliations

Determining the concentration and the absorptivity factor at 260 nm in sodium dodecyl sulfate of the adenovirus reference material using analytical ultracentrifugation

Steven A Berkowitz. Anal Biochem. .

Abstract

A concentration of 6.4 +/- 0.2 (at 1 SD) x 10(11) virus particles (vp) per milliliter was determined for the adenovirus reference material (ARM) by averaging analytical ultracentrifugation concentration data obtained with refractometric detection with the completely independent concentration reported by the Adenoviral Reference Material Working Group (ARMWG). Using this concentration, the ARM absorptivity factor (in sodium dodecyl sulfate [SDS] at 260 nm) of 1.2+/-0.1 (at 1 SD) x 10(12) vp/ml per OD(260 nm) per centimeter was obtained.

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