Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2008 Jul 9;582(16):2352-6.
doi: 10.1016/j.febslet.2008.05.045. Epub 2008 Jun 6.

HemK2 protein, encoded on human chromosome 21, methylates translation termination factor eRF1

Sabine Figaro  1 , Nathalie Scrima, Richard H Buckingham, Valérie Heurgué-Hamard
Affiliations
Free article

HemK2 protein, encoded on human chromosome 21, methylates translation termination factor eRF1

Sabine Figaro et al. FEBS Lett. .
Free article

Abstract

The ubiquitous tripeptide Gly-Gly-Gln in class 1 polypeptide release factors triggers polypeptide release on ribosomes. The Gln residue in both bacterial and yeast release factors is N5-methylated, despite their distinct evolutionary origin. Methylation of eRF1 in yeast is performed by the heterodimeric methyltransferase (MTase) Mtq2p/Trm112p, and requires eRF3 and GTP. Homologues of yeast Mtq2p and Trm112p are found in man, annotated as an N6-DNA-methyltransferase and of unknown function. Here we show that the human proteins methylate human and yeast eRF1.eRF3.GTP in vitro, and that the MTase catalytic subunit can complement the growth defect of yeast strains deleted for mtq2.

PubMed Disclaimer

Publication types

MeSH terms