Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2008 Jul 4;26(29-30):3608-16.
doi: 10.1016/j.vaccine.2008.04.074. Epub 2008 May 19.

Evaluation of systemic and mucosal anti-HPV16 and anti-HPV18 antibody responses from vaccinated women

Troy J Kemp  1 , Alfonso García-Piñeres, Roni T Falk, Sylviane Poncelet, Francis Dessy, Sandra L Giannini, Ana Cecilia Rodriguez, Carolina Porras, Rolando Herrero, Allan Hildesheim, Ligia A Pinto; Costa Rica Vaccine Trial (CVT) Group
Collaborators, Affiliations

Evaluation of systemic and mucosal anti-HPV16 and anti-HPV18 antibody responses from vaccinated women

Troy J Kemp et al. Vaccine. .

Abstract

Ideal methods to monitor HPV neutralizing antibodies induced by vaccination have not been established yet. Here, we evaluated systemic and cervical antibody levels induced by HPV16/18 AS04-adjuvanted vaccine (GlaxoSmithKline Biologicals) using a secreted alkaline phosphatase neutralization assay (SEAP-NA) and enzyme-linked immunosorbent assay (ELISA). Serum and cervical secretions from 50 vaccinated women were used to assess (1) overall assay reproducibility; (2) inter-assay and inter-specimen correlation; (3) correlations between month 1 and month 12 titers. Strong correlations between SEAP-NA and ELISA were observed (serum anti-HPV16/18, rho=0.91/0.85; cervix anti-HPV16/18, rho=0.84/0.89). Systemic and cervical antibody measures also correlated well (rho range: 0.64-0.75); except at mid-cycle (rho range: 0.28-0.65). Correlations between antibody levels at 1 and 12 months following the start of vaccination were poor (rho range: 0.16-0.38). In conclusion, HPV16/18 VLP-based ELISA is a reliable and valid method to monitor anti-HPV16/18 neutralizing potential for the first year following vaccination; however, additional studies will be required to better define the effects of the time on cycle and patterns of antibody response over time following vaccination.

PubMed Disclaimer

Figures

Figure 1
Figure 1
SEAP-NA and ELISA anti-HPV16 and 18 titers were correlated with each other among serum and cervical secretions. (A) HPV16 SEAP-NA correlated with HPV16 ELISA using serum samples. (B) HPV18 SEAP-NA correlated with HPV18 ELISA using serum samples. (C) HPV16 SEAP-NA correlated with HPV16 ELISA using cervical secretions. (D) HPV18 SEAP-NA correlated with HPV18 ELISA using cervical secretions. Within each graph the total number of samples used in the analysis is represented by ‘N’, and the Spearman rank correlation coefficient and p-value is also indicated on each graph.
Figure 2
Figure 2
Correlations calculated between serum anti-HPV16 and 18 antibody responses and cervical secretion anti-HPV16 and 18 antibody responses. (A) Serum anti-HPV16 antibody titers correlated with cervical secretion anti-HPV16 antibody titers using the HPV16 L1 VLP-based ELISA. (B) Serum anti-HPV18 antibody titers correlated with cervical secretion anti-HPV18 antibody titers using the HPV18 L1 VLP-based ELISA. (C) Serum anti-HPV16 antibody titers correlated with cervical secretion anti-HPV16 antibody titers using the SEAP-NA. (D) Serum anti-HPV18 antibody titers correlated with cervical secretion anti-HPV18 antibody titers using the SEAP-NA. Within each graph the total number of samples used in the analysis is represented by ‘N’, and the Spearman rank correlation coefficient and p-value is also indicated on each graph.
Figure 3
Figure 3
Anti-HPV16 and 18 antibody responses were correlated with each other within the same assay and specimen type. (A) Serum anti-HPV16 antibody titers were correlated with serum anti-HPV18 antibody titers using the titers calculated from the ELISA. (B) Serum anti-HPV16 antibody titers were correlated with serum anti-HPV18 antibody titers using the titers calculated from the SEAP-NA. (C) Cervical secretion anti-HPV16 antibody titers were correlated with cervical secretion anti-HPV18 antibody titers calculated from the ELISA results. (D) Cervical secretion anti-HPV16 antibody titers were correlated with cervical secretion anti-HPV18 antibody titers calculated from the SEAP-NA results. Within each graph the total number of samples used in the analysis is represented by ‘N’, and the Spearman rank correlation coefficient and p-value is also indicated on each graph.
See this image and copyright information in PMC

References

    1. Munoz N, Bosch FX, de Sanjose S, Herrero R, Castellsague X, Shah KV, et al. Epidemiologic classification of human papillomavirus types associated with cervical cancer. N Engl J Med. 2003;348(6):518–27. - PubMed
    1. Cogliano V, Baan R, Straif K, Grosse Y, Secretan B, El Ghissassi F. Carcinogenicity of human papillomaviruses. Lancet Oncol. 2005;6(4):204. - PubMed
    1. Bosch FX, Manos MM, Munoz N, Sherman M, Jansen AM, Peto J, et al. Prevalence of human papillomavirus in cervical cancer: a worldwide perspective. International biological study on cervical cancer (IBSCC) Study Group. J Natl Cancer Inst. 1995;87(11):796–802. - PubMed
    1. Munoz N, Bosch FX, Castellsague X, Diaz M, de Sanjose S, Hammouda D, et al. Against which human papillomavirus types shall we vaccinate and screen? The international perspective. Int J Cancer. 2004;111(2):278–85. - PubMed
    1. Castellsague X, Diaz M, de Sanjose S, Munoz N, Herrero R, Franceschi S, et al. Worldwide human papillomavirus etiology of cervical adenocarcinoma and its cofactors: implications for screening and prevention. J Natl Cancer Inst. 2006;98(5):303–15. - PubMed

Publication types

MeSH terms