doi: 10.1128/JVI.20.2.527-531.1976.
Anatomy of herpes simplex virus DNA. VI. Defective DNA originates from the S component
- PMID: 185428
- PMCID: PMC355020
- DOI: 10.1128/JVI.20.2.527-531.1976
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Anatomy of herpes simplex virus DNA. VI. Defective DNA originates from the S component
J Virol.
1976 Nov.
Abstract
We previously reported that serial propagation of the Justin strain of herpes simplex virus 1 [HSV-1 (Justin)] results in the generation of defective DNA molecules consisting of tandem repetitions of sequences of limited complexity. In the present study, HSV-1 DNA was cleaved with the restriction endonucleases BglII and EcoRI. The fragments were electrophoretically separated on agarose gels, transferred to nitrocellulose strips, and then hybridized with 32P-labeled HSV-1 (Justin) defective DNA. The data allow us to conclude that DNA sequences contained in the repeat unit of defective DNA originate from the S segment of the wild-type viral DNA molecule.
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