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Review
. 2008 Jun 15;14(12):3699-705.
doi: 10.1158/1078-0432.CCR-07-4733.

Tissue-based research in kidney cancer: current challenges and future directions

Sabina Signoretti  1 , Gennady Bratslavsky, Frederick M Waldman, Victor E Reuter, John Haaga, Maria Merino, George V Thomas, Michael R Pins, Towia Libermann, John Gillespie, Joseph E Tomaszewski, Carolyn C Compton, Andrew Hruszkewycz, W Marston Linehan, Michael B Atkins
Affiliations
Review

Tissue-based research in kidney cancer: current challenges and future directions

Sabina Signoretti et al. Clin Cancer Res. .

Abstract

The past several years have seen unprecedented advances in the application of various therapeutic strategies for the treatment of patients with renal cancer. The availability of active immunotherapy, antiangiogenic therapy, and targeted therapy for this disease has brought front and center issues related to choosing the appropriate treatment for particular patient populations. It is increasingly evident that the most promising treatment selection strategies will incorporate identifying specific features of the tumor itself. To facilitate this move toward personalized medicine, it is critically important to establish some standard principles for renal cancer tissue collection, preparation, and analysis for translational research studies. In this article, we identify and discuss some critical issues related to tissue-based kidney cancer research. We focus on five major areas as follows: (a) surgical and image-guided techniques for tissue collection; (b) quality control of specimen collection, processing, storage, and review; (c) issues related to analysis of paraffin embedded tissues; (d) genomic studies; and (e) assessment of reproducibility of assays across institutions. In addition, some practical implementation strategies are proposed. Although many of the topics discussed are specific for renal cancer, several are also relevant to tissue based biomarker investigations in a broad array of malignancies.

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Figures

Figure 1
Figure 1
Variables recorded in the NCI Tissue Procurement Form include clinical diagnosis, specimen site, type of anesthesia, induction/anesthesia start time, incision time, type of surgery (open versus laparoscopic), ischemia time, type of ischemia (warm versus cold), and resection time. Specific tumor characteristics such as size and macroscopic features are also included.
Figure 2
Figure 2
Schematic of the experimental design utilized by Dr. Bratslavsky to assess the impact of storage temperature and time on mRNA and protein expression in renal cancer tissue.
See this image and copyright information in PMC

References

    1. Atkins MB, Ernstoff MS, Figlin RA, Flaherty KT, George DJ, Kaelin WG, Jr, et al. Innovations and challenges in renal cell carcinoma: summary statement from the Second Cambridge Conference. Clin Cancer Res. 2007 Jan 15;13(2 Pt 2):667s–70s. - PubMed
    1. Brugarolas J. Renal-cell carcinoma--molecular pathways and therapies. N Engl J Med. 2007 Jan 11;356(2):185–7. - PubMed
    1. Lechevallier E, Andre M, Barriol D, Daniel L, Eghazarian C, De Fromont M, et al. Fine-needle percutaneous biopsy of renal masses with helical CT guidance. Radiology. 2000 Aug;216(2):506–10. - PubMed
    1. Rybicki FJ, Shu KM, Cibas ES, Fielding JR, vanSonnenberg E, Silverman SG. Percutaneous biopsy of renal masses: sensitivity and negative predictive value stratified by clinical setting and size of masses. AJR Am J Roentgenol. 2003 May;180(5):1281–7. - PubMed
    1. Volpe A, Kachura JR, Geddie WR, Evans AJ, Gharajeh A, Saravanan A, et al. Techniques, safety and accuracy of sampling of renal tumors by fine needle aspiration and core biopsy. J Urol. 2007 Aug;178(2):379–86. - PubMed

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