Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1976 Oct;73(10):3438-42.
doi: 10.1073/pnas.73.10.3438.

Novel structure at 5'-ends of nascent DNA chains

D W Siegmann, R Werner

Novel structure at 5'-ends of nascent DNA chains

D W Siegmann et al. Proc Natl Acad Sci U S A. 1976 Oct.

Abstract

Because of their association with protein short nascent DNA chains in Escherichia coli can be separated from other cellular DNA by chromatography on hydroxylapatite. Protein-free DNA chains of less than 500 nucleotides in length are resistant to degradation from the 5'-end by alkaline phosphatase [orthophosphoric-monoester phosphohydrolase (alkaline optimum); EC 3.1.3.1] and spleen phosphodiesterase (oligonucleate 3'-nucleotidohydrolase; EC 3.1.4.18). In contrast, DNA chains containing more than 500 nucleotides are degradable. From these results we conclude that short nascent DNA chains are structurally modified at their 5'-ends. The nature of this structure and its possible functions are discussed.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Proc Natl Acad Sci U S A. 1975 Jan;72(1):362-6 - PubMed
    1. Proc Natl Acad Sci U S A. 1969 Dec;64(4):1242-8 - PubMed
    1. J Mol Biol. 1975 Feb 15;92(1):145-63 - PubMed
    1. J Mol Biol. 1975 Jun 15;95(1):45-61 - PubMed
    1. J Mol Biol. 1975 Jun 15;95(1):63-70 - PubMed