Expression of Foxp4 in the developing and adult rat forebrain

Abstract

Many members of the Fox family are transcription factors that regulate the morphogenesis of various organs. In the present study, we examined the expression pattern of Foxp4, a member of the Foxp subfamily, and compared its pattern with the patterns of Foxp2 and Foxp1 in the developing rat brain. In general, these three Foxp genes shared partially overlapping and yet differentially regulated expression patterns in the striatum, the cerebral cortex, and the thalamus during development. In the developing dorsal telencephalon, a mediolateral gradient of Foxp4 was present in the cortical primordium, with high levels in the ventricular zone of the medial cortex. By contrast, no gradient of Foxp2 and Foxp1 was detected in the dorsal telencephalon. At later stages, Foxp4 was expressed throughout all cortical layers as opposed to the layer-specific expression of Foxp2 and Foxp1. In the developing striatum, the pattern of Foxp4 expression was distinct from the patterns of Foxp2 and Foxp1. The spatial expression pattern of Foxp4 was similar to that of Foxp2 during the early embryonic stage. However, from the late embryonic stage to postnatal day 4, Foxp4 was expressed in a mediolateral gradient and decreased in the striosomal compartment, in contrast to the striosomal expression of Foxp2 and homogeneous expression of Foxp1. Foxp4 was developmentally down-regulated such that Foxp4 was undetectable in the forebrain after postnatal day 14, whereas Foxp2 and Foxp1 were persistently expressed in adulthood. Given that Foxp4, Foxp2, and Foxp1 are capable of heterodimerization for transcriptional regulation, the partially overlapping expression patterns of Foxp4, Foxp2, and Foxp1 in different domains of the developing forebrain suggest that each member and/or different combinatory actions of the Foxp subfamily may play a pivotal role in regulating forebrain development.