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. 1976 Oct;73(10):3539-43.
doi: 10.1073/pnas.73.10.3539.

Partial purification and characterization of the mRNA for rat preproinsulin

J R Duguid, D F Steiner, W L Chick

Partial purification and characterization of the mRNA for rat preproinsulin

J R Duguid et al. Proc Natl Acad Sci U S A. 1976 Oct.

Abstract

Electrophoretically homogeneous messenger RNA for rat preproinsulin has been prepared from an islet cell tumor by the use of oligo(dT(-cellulose chromatography and sucrose density gradient centrifugation. The molecular weight of the mRNA is about 210,000, as determined by polyacrylamide gel electrophoresis in formamide, and its sedimentation coefficient is 9.3 S in sucrose gradients containing 0.2 M NaCl. These results indicate that the synthesis of preproinsulin is directed by a monocistronic mRNA 600 nucleotides in length and requires approximately 55% of the molecule's coding capacity.

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References

    1. Nucleic Acids Res. 1976 Feb;3(2):381-91 - PubMed
    1. Nature. 1975 May 1;255(5503):33-7 - PubMed
    1. Anal Biochem. 1971 Feb;39(2):462-77 - PubMed
    1. Proc Soc Exp Biol Med. 1971 May;137(1):280-3 - PubMed
    1. Nature. 1974 Nov 29;252(5482):354-9 - PubMed

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