doi: 10.1073/pnas.73.10.3539.
Partial purification and characterization of the mRNA for rat preproinsulin
- PMID: 185618
- PMCID: PMC431152
- DOI: 10.1073/pnas.73.10.3539
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Partial purification and characterization of the mRNA for rat preproinsulin
Proc Natl Acad Sci U S A.
1976 Oct.
Abstract
Electrophoretically homogeneous messenger RNA for rat preproinsulin has been prepared from an islet cell tumor by the use of oligo(dT(-cellulose chromatography and sucrose density gradient centrifugation. The molecular weight of the mRNA is about 210,000, as determined by polyacrylamide gel electrophoresis in formamide, and its sedimentation coefficient is 9.3 S in sucrose gradients containing 0.2 M NaCl. These results indicate that the synthesis of preproinsulin is directed by a monocistronic mRNA 600 nucleotides in length and requires approximately 55% of the molecule's coding capacity.
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