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. 2008 Jun;28(3):343-5.
doi: 10.1007/s11596-008-0326-z. Epub 2008 Jun 19.

Immune killing activity of lymphocytes on Hela cells expressing interleukin-12 in vitro

Huiyan Wang  1 , Suhua Chen
Affiliations

Immune killing activity of lymphocytes on Hela cells expressing interleukin-12 in vitro

Huiyan Wang et al. J Huazhong Univ Sci Technolog Med Sci. 2008 Jun.

Abstract

The killing effects of lymphocytes on Hela cells expressing interleukin-12 (IL-12) in vitro were explored. By using gene transfection technique, full length IL-12 gene was transfected into Hela cells. The expression of IL-12 in Hela cells was detected quantitatively by ELISA; Changes in killing effects of lymphocytes on Hela cells expressing IL-12 were observed by MTT. It was found that Hela cells could express IL-12 between 24 h and 72 h after transfection. Killing activity of lymphocytes on Hela cells expressing IL-12 was significantly enhanced. It was concluded by cell transfection technique, Hela cells could express IL-12 and were more easily killed by lymphocytes.

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