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. 2008:2008:168652.
doi: 10.1155/2008/168652.

Lipoperoxidation and protein oxidative damage exhibit different kinetics during septic shock

Max Andresen  1 , Tomas Regueira, Alejandro Bruhn, Druso Perez, Pablo Strobel, Alberto Dougnac, Guillermo Marshall, Federico Leighton
Affiliations

Lipoperoxidation and protein oxidative damage exhibit different kinetics during septic shock

Max Andresen et al. Mediators Inflamm. 2008.

Abstract

Septic shock (SS)-related multiorgan dysfunction has been associated with oxidative damage, but little is known about the temporal damage profile and its relationship to severity. The present work investigated prospectively 21 SS patients. Blood samples were obtained at diagnosis, 24, 72 hours, day 7, and at 3 months. At admission, thiobarbituric acid reactive substances (TBARSs), plasma protein carbonyls, plasma protein methionine sulfoxide (MS), ferric/reducing antioxidant power (FRAP), total red blood cell glutathione (RBCG), uric acid (UA), and bilirrubin levels were increased (P < .05). Total radical-trapping antioxidant potential (TRAP) and vitamin-E were similar to controls, and vitamin-C was decreased (P < .05). During evolution, TBARS and RBCG increased (P < .001), vitamin-E levels remained stable, whereas plasma protein carbonyls and MS, TRAP, vitamin-C, reduced glutathione, and UA levels decreased (P < .006). After 3 months, plasma protein carbonyls and MS persisted elevated. More severe patients exhibited higher TBARS, TRAP, FRAP, vitamin-C, UA, and bilirrubin levels. Our results suggest early and persistent oxidative stress during septic shock and a correlation between increasing levels of lipoperoxidation and sepsis severity.

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Figures

Figure 1
Figure 1
Temporal evolution of oxidative damage of all septic shock patients in (a) lipoperoxidation (TBARS), (b) carbonyls, and (c) Methionine sulfoxide. Normal values (mean ± SD) obtained from matched healthy subjects are shown as continuous and dotted lines, respectively. p: reflects variation in time calculated by linear mixed effects model.*Indicates significant differences between normal values and patients at different time points (t-test for independent samples).
Figure 2
Figure 2
Evolution of antioxidant levels in all septic shock patients of (a) Vitamin C, (b) Vitamin E (alpha-Tocopherol), and (c) reduced and oxidized glutathione. Normal values (mean ± SD) obtained from matched healthy subjects are shown as continuous and dotted lines, respectively. p: reflects variation in time calculated by linear mixed effects model.*Indicates significant differences between normal values and patients at different time points (t-test for independent samples).
Figure 3
Figure 3
Correlations of peak FRAP and TRAP levels with peak uric acid levels. P < .05 for both correlations exposed.
Figure 4
Figure 4
Admission correlations of peak SOFA score and peak lactate levels with peak FRAP levels. P < .05 for the correlation exposed.
Figure 5
Figure 5
Evolution correlations of peak SOFA score and peak lactate levels with (a) peak TBARS levels and (b) peak FRAP levels. P < .05 for all correlations exposed.
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