[Characterization of Triticum aestivum-Dasypyrum villosum germplasms Shannong05078]

Abstract

A germplasm line Shannong05078 was developed from the progenies of Am3 x Triticum aestivum-Dasypyrum villosum amphidiploid, the purpose of this study is to ascertain the genome composition of Shannong05078 and then supply wheat breeding with reference. The results showed that Shannong05078 was stable in morphology characters and presenting favorable agronomic traits, it was resistant to powdery mildew and rust. Its protein content was higher, and the HWM-GS of it was 7+8, 5+10. The chromosome number of Shannong05078 was 2n=28, and the chromosome configuration was 2n=14 II at the PMC MI. SSR analysis showed that Shannong05078 contained the whole A and B genomes, and had little genetic materials from D genome. A specific primer of Dasypyrum villosum (phv29) was used to identify the genetic materials form Dasypyrum villosum in Shannong05078, and specific band of Dasypyrum villosum appeared in it. It can be deduced that the genomic composition of Shannong05078 is 2n=AABB=28, and genetic materials of D genome and Dasypyrum villosum were translated into Shannong05078.