Effects of resistant starch on colonic preneoplastic aberrant crypt foci in rats

Abstract

This study was designed to determine the effects of resistant starch (RS) at different levels on the azoxymethane (AOM)-induced colonic aberrant crypt foci (ACF) at the pre-initiation (PI) and promotion (P) stages in Wistar rats. According to the consuming assigned diets, all animals received AOM at a dosage of 15 mg/kg once a week for two consecutive weeks. In experiment 1, four groups of rats (n=12) were given AOM after 3 weeks of consuming the AIN-76 (control group) and RS diets. In experiment 2, four groups of rats (n=12) were given AOM before 3 weeks of consuming the AIN-76 and RS diets. Rats were killed after 13 weeks of initial injecting AOM. Colons were fixed in formalin and ACF were quantified after staining. In experiment 1, rats fed RS had more ACF than that of the control fed rats. In experiment 2, rats fed RS had fewer ACF than that of the control fed rats. The results indicate that dietary RS suppresses AOM-induced ACF formation, only at the P stage. A significant dose-response effect was observed between suppression of ACF formation and dietary RS amount. However, RS promote the formation of ACF at the PI stage.