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. 2008 Jun;9(3):177-83.
doi: 10.1080/17482960801933942.

Oxidative stress biomarkers in sporadic ALS

Hiroshi Mitsumoto  1 , Regina M Santella, Xinhua Liu, Mikhail Bogdanov, Jennifer Zipprich, Hui-Chen Wu, Julie Mahata, Mary Kilty, Kate Bednarz, Daniel Bell, Paul H Gordon, Mady Hornig, Mahsa Mehrazin, Ali Naini, M Flint Beal, Pam Factor-Litvak
Affiliations

Oxidative stress biomarkers in sporadic ALS

Hiroshi Mitsumoto et al. Amyotroph Lateral Scler. 2008 Jun.

Abstract

We aimed to investigate oxidative stress biomarkers in a cross-sectional pilot study of 50 participants with sporadic ALS (SALS) compared to 46 control subjects. We measured urinary 8-oxodeoxyguanosine (8-oxodG), urinary 15-F(2t)-isoprostane (IsoP), and plasma protein carbonyl by ELISA methods. We also determined if ELISA measurement of 8-oxodG could be validated against measures from high-pressure liquid chromatography coupled with electrochemical detection, the current standard method. We found that 8-oxodG and IsoP levels adjusted for creatinine were significantly elevated in SALS participants. These differences persisted after age and gender were controlled in regression analyses. These markers are highly and positively correlated with each other. 8-oxodG measured by the two techniques from the same urine sample were positively correlated (p<.0001). Protein carbonyl was not different between SALS participants and controls. In conclusion, using ELISA, we confirmed that certain oxidative stress biomarkers were elevated in SALS participants. ELISA may be reliable and thus useful in epidemiology studies requiring large numbers of samples to determine the significance of increased oxidative stress markers in SALS. Further studies are required.

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Conflict of interest statement

Conflict of Interest Statement: None of the authors have conflicts of interests in relation to the presented study. HM received a research grant from Aeolus, served as a consultant for Eisai Pharma, received an honorarium for an advisory board meeting for Teva, and received educational grants for a CME course at Columbia from Sanofi-Aventis, Avenia, Ethena, and Bioscrips.

Figures

Figure 1
Figure 1
Data distributions for urinary oxidative stress markers (adjusted for creatinine): a) 8-oxodG measured by ELISA, b) isoprostane measured by ELISA, and c) 8-oxodG measured by HPLC/ECD. For each boxplot, the middle dark line represents the median. The shaded box represents the middle two quartiles (Q2 and Q3) of the data. The brackets at the end of the dashed lines represent the data point closest to and within 150% of the Q2/Q3 data span. The dots represent outlier values.
Figure 2
Figure 2
Correlation of 8-oxodG, measured by ELISA and by high pressure liquid chromatography coupled with electrochemical detector (HPLC/ECD) in the same urine samples.
See this image and copyright information in PMC

References

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