Tracing back EFL gene evolution in the cryptomonads-haptophytes assemblage: separate origins of EFL genes in haptophytes, photosynthetic cryptomonads, and goniomonads

Abstract

A recently identified GTPase, elongation factor-like (EFL) protein is proposed to bear the principal functions of translation elongation factor 1alpha (EF-1alpha). Pioneering studies of EF-1alpha/EFL evolution have revealed the phylogenetically scattered distribution of EFL amongst eukaryotes, suggesting frequent eukaryote-to-eukaryote EFL gene transfer events and subsequent replacements of EF-1alpha functions by EFL. We here determined/identified seven new EFL sequences of the photosynthetic cryptomonad Cryptomonas ovata, the non-photosynthetic cryptomonad (goniomonad) Goniomonas amphinema, the foraminifer Planoglabratella opecularis, the haptophyte Chrysochromulina sp., the centroheliozoan Raphidiophrys contractilis, and two red algae Chondrus crispus and Gracilaria changii. The analyses of these EFL sequences successfully brought new insights into lateral EFL gene transfer amongst eukaryotes. Of most interest is a complex EFL evolution in a monophyletic assemblage comprised of cryptomonads and haptophytes. Since our analyses rejected any phylogenetic affinity amongst the EFL sequences from Goniomonas, photosynthetic cryptomonads, and haptophytes, the EFL genes of the three lineages most likely originated from different phylogenetic sources.