Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 2008 Jul;67(1):1-12.
doi: 10.1007/s00239-008-9103-7. Epub 2008 Jul 2.

Comparative analysis of the glg operons of Pectobacterium chrysanthemi PY35 and other prokaryotes

Kye Man Cho  1 , Woo Jin Lim, Renukaradhya K Math, Shah Md Asraful Islam, Sun Joo Hong, Hoon Kim, Han Dae Yun
Affiliations
Comparative Study

Comparative analysis of the glg operons of Pectobacterium chrysanthemi PY35 and other prokaryotes

Kye Man Cho et al. J Mol Evol. 2008 Jul.

Abstract

A chromosomal region of Pectobacterium chrysanthemi PY35 that contains of genes for glycogen synthesis was isolated from a cosmid library. The operon consists of glycogen branching enzyme (glgB), glycogen debranching enzyme (glgX), ADP-glucose pyrophosphorylase (glgC), glycogen synthase (glgA), and glycogen phosphorylase (glgP) genes. Gene organization is similar to that of Escherichia coli. The purified ADP-glucose pyrophosphorylase (GlgC) was activated by fructose 1,6-bisphosphate and inhibited by AMP. The constructed glgX::Omega mutant failed to integrate into the chromosome of P. chrysanthemi by marker exchange. Phylogenetic analysis based on the 16S rDNA and the amino acid sequence of Glg enzymes showed correlation with other bacteria. gamma-Proteobacteria have the glgX gene instead of the bacilli glgD gene in the glg operon. The possible evolutionary implications of the results among the prokaryotes are discussed.

PubMed Disclaimer

References

    1. J Bacteriol. 2006 Jul;188(14):5266-72 - PubMed
    1. Annu Rev Microbiol. 1994;48:53-80 - PubMed
    1. DNA Seq. 1993;4(1):1-9 - PubMed
    1. J Bacteriol. 1998 Dec;180(24):6557-64 - PubMed
    1. J Mol Evol. 2005 Feb;60(2):257-67 - PubMed

Publication types

MeSH terms

LinkOut - more resources