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. 2008 Jun 19;13(6):1372-83.
doi: 10.3390/molecules13061372.

Fractionated marine invertebrate extract libraries for drug discovery

Tim S Bugni  1 , Mary Kay Harper, Malcolm W B McCulloch, Jason Reppart, Chris M Ireland
Affiliations

Fractionated marine invertebrate extract libraries for drug discovery

Tim S Bugni et al. Molecules. .

Abstract

The high-throughput screening and drug discovery paradigm has necessitated a change in preparation of natural product samples for screening programs. In an attempt to improve the quality of marine natural products samples for screening, several fractionation strategies were investigated. The final method used HP20SS as a solid support to effectively desalt extracts and fractionate the organic components. Additionally, methods to integrate an automated LCMS fractionation approach to shorten discovery time lines have been implemented.

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Figures

Figure 1
Figure 1
Example of HP20SS library storage.
Figure 2
Figure 2
LCMS chromatogram showing effective separation of pyridinium polymers.
Figure 3
Figure 3
Overall scheme for pursuing assay hits.
Figure 4
Figure 4
Comparison of chromatography on a 3 mm ID column (A) and a 4.6 ID column (B).
See this image and copyright information in PMC

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