Erythrocytic oxidative damage in crossbred cattle naturally infected with Babesia bigemina

Abstract

This study aimed to determine the erythrocytic lipid peroxidation and haemoglobin oxidation as contributory factors causing anaemia in cattle (Friesian x Egyptian native breed) infected with Babesia bigemina. Blood was collected from 32 cows infected with B. bigemina along with 18 healthy cows as controls for determination of erythrocytic malondialdehyde (MDA), blood methaemoglobin (MetHb), plasma free haemoglobin (PHb), corpuscular osmotic fragility (COF), red blood cell count (RBC), total haemoglobin (Hb) and packed cell volume (PCV). Percentage of parasitaemia varied from 14% to 36%. MDA, MetHb, COF and PHb were significantly increased (P<0.001) in infected cows versus controls. Parasitaemia was positively correlated (P<0.001) with MDA, MetHb, COF and PHb. MDA was positively correlated (P<0.001) with COF and PHb and negatively correlated (P<0.001) with RBC, Hb and PCV. MetHb was negatively correlated (P<0.001) with RBC, Hb and PCV and positively correlated (P<0.001) with COF. In conclusion, B. bigemina infection in cattle is associated with a parasitic burden-dependent corpuscular oxidative damage as indicated by membrane lipid peroxidation and methaemoglobin formation, which are contributed to COF and intravascular haemolysis.