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. 1992 Feb 20;39(4):447-9.
doi: 10.1002/bit.260390411.

Cell immobilization using PVA crosslinked with boric acid

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Cell immobilization using PVA crosslinked with boric acid

K Y Wu et al. Biotechnol Bioeng. .

Abstract

A new cell immobilization technique is described in which polyvinyl alcohol is crosslinked with boric acid, with the addition of a small amount of calcium alginate. The presence of the calcium alginate improves the surface properties of the beads, preventing agglomeration. A pure culture of phenol-degrading Pseudomonas was immobilized in the PVA-alginate beads. Phenol was successfully degraded in a fluidized bed of the beads, indicating that cell viability was maintained following the immobilization procedure. The PVA-alginate beads proved to be very strong and durable, with no noticeable degradation of the beads after 2 weeks of continuous operation of the fluidized bed.

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